Document Detail


Isolation of UV-sensitive mutants of mouse L5178Y cells by a cell suspension spotting method.
MedLine Citation:
PMID:  7112349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have isolated 56 UV-sensitive mutant clones from a mouse L51 T/t line of L5178Y cells by a cell suspension spotting method. Five mutants have also been isolated from L51 T/t and L5178Y cells by the method reported by Thompson and coworkers (22). We divided the mutants into two groups, "highly sensitive" and "moderately sensitive" mutants, according to their sensitivity to UV irradiation. Fifty-eight mutants were highly sensitive and three were moderately sensitive to UV. The reconstruction experiments indicate that more than 90% of highly sensitive mutants were recovered by the cell suspension spotting method. Frequencies of recovered mutants highly sensitive to UV increased with increasing dose of mutagens. Recovered mutant frequency reached 10(-2) after treatment with 1.5 micrograms/ml of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) (survival 0.2%). Eight UV-sensitive mutants were divided into four complementation groups. These mutants were 2-6 times more sensitive to UV than parental L51 T/t cells in terms of D37 (dose required to reduce survival to 37%). Four representative UV-sensitive mutants which are classified into different complementation groups were examined for their sensitivity to killing by UV, 4-nitroquinoline-1-oxide (4NQO), mitomycin C (MMC), X-rays, and MNNG. All four classes of mutants were found to be cross-sensitive to UV, 4NQO, and MMC, but not sensitive to X-rays and MNNG.
Authors:
T Shiomi; N Hieda-Shiomi; K Sato
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Somatic cell genetics     Volume:  8     ISSN:  0098-0366     ISO Abbreviation:  Somatic Cell Genet.     Publication Date:  1982 May 
Date Detail:
Created Date:  1982-10-12     Completed Date:  1982-10-12     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7506054     Medline TA:  Somatic Cell Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  329-45     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects,  radiation effects
Clone Cells
Ethyl Methanesulfonate / pharmacology
Genetic Complementation Test
Leukemia L5178 / physiopathology*
Leukemia, Experimental / physiopathology*
Methods
Methylnitronitrosoguanidine / pharmacology
Mice
Mutation
Ultraviolet Rays*
Chemical
Reg. No./Substance:
62-50-0/Ethyl Methanesulfonate; 70-25-7/Methylnitronitrosoguanidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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