| Isolation of Trypanosoma cruzi from the blood of infected mice by column chromatography. | |
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MedLine Citation:
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PMID: 375218 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trypomastogotes of three strains of Trypanosoma cruzi were isolated from the blood of infected mice employing lymphoprep for separation of the red blood cells and a column of DEAE cellulose for removal of white cells and platelets. An average recovery of 45 to 58 percent of actively motile, infective organisms, free of contaminating blood cells was obtained. Protein and carbohydrate assays of the separated organisms revealed significant differences between the Tulahuen, a reticulotropic strain, and the House 510 and House 11, two myotropic strains of this parasitic species. The present procedure should provide sufficient parasites for physiological and biochemical studies; it has also served to indicate particular strain characteristics which may aid in a taxonomic classification of these organisms. |
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Authors:
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T I Mercado; K Katusha |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Preparative biochemistry Volume: 9 ISSN: 0032-7484 ISO Abbreviation: Prep. Biochem. Publication Date: 1979 |
Date Detail:
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Created Date: 1979-07-28 Completed Date: 1979-07-28 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 1276634 Medline TA: Prep Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 97-106 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood / parasitology Carbohydrates / analysis Chromatography, DEAE-Cellulose Male Mice Proteins / analysis Species Specificity Trypanosoma cruzi / analysis, classification, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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