Document Detail


Isolation of Trypanosoma cruzi from the blood of infected mice by column chromatography.
MedLine Citation:
PMID:  375218     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trypomastogotes of three strains of Trypanosoma cruzi were isolated from the blood of infected mice employing lymphoprep for separation of the red blood cells and a column of DEAE cellulose for removal of white cells and platelets. An average recovery of 45 to 58 percent of actively motile, infective organisms, free of contaminating blood cells was obtained. Protein and carbohydrate assays of the separated organisms revealed significant differences between the Tulahuen, a reticulotropic strain, and the House 510 and House 11, two myotropic strains of this parasitic species. The present procedure should provide sufficient parasites for physiological and biochemical studies; it has also served to indicate particular strain characteristics which may aid in a taxonomic classification of these organisms.
Authors:
T I Mercado; K Katusha
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Preparative biochemistry     Volume:  9     ISSN:  0032-7484     ISO Abbreviation:  Prep. Biochem.     Publication Date:  1979  
Date Detail:
Created Date:  1979-07-28     Completed Date:  1979-07-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  1276634     Medline TA:  Prep Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  97-106     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood / parasitology
Carbohydrates / analysis
Chromatography, DEAE-Cellulose
Male
Mice
Proteins / analysis
Species Specificity
Trypanosoma cruzi / analysis,  classification,  isolation & purification*
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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