Document Detail


Isolation of T cells using rosetting procedures.
MedLine Citation:
PMID:  18432833     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This unit describes a procedure for separating T cells from other mononuclear cells by exploiting the unique ability of cells to bind to and form rosettes with sheep red blood cells (SRBC). This isolation method also allows recovery of the nonrosetting cell population (B lymphocytes, monocytes, and macrophages). Neuraminidase- and 2 -aminoethylisothiouronium bromide (AET)-treated SRBC are used for rosetting because of enhanced binding to T cells.
Authors:
M E Kanof
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in immunology / edited by John E. Coligan ... [et al.]     Volume:  Chapter 7     ISSN:  1934-368X     ISO Abbreviation:  Curr Protoc Immunol     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-04-24     Completed Date:  2008-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9101651     Medline TA:  Curr Protoc Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 7.2     Citation Subset:  IM    
Affiliation:
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Humans
Rosette Formation / methods*
T-Lymphocytes / cytology*,  immunology

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