| Isolation of T cells using rosetting procedures. | |
| | |
MedLine Citation:
|
PMID: 18432833 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This unit describes a procedure for separating T cells from other mononuclear cells by exploiting the unique ability of cells to bind to and form rosettes with sheep red blood cells (SRBC). This isolation method also allows recovery of the nonrosetting cell population (B lymphocytes, monocytes, and macrophages). Neuraminidase- and 2 -aminoethylisothiouronium bromide (AET)-treated SRBC are used for rosetting because of enhanced binding to T cells. |
| | |
Authors:
|
M E Kanof |
Related Documents
:
|
9347653 - Control cells for both electrical impedance and light scattering automated hematologica... 19445823 - Comparison of the activity of human and bovine milk on two cell lines. 3417053 - Cell surface proteoglycan expression correlates with epithelial-mesenchymal interaction... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Current protocols in immunology / edited by John E. Coligan ... [et al.] Volume: Chapter 7 ISSN: 1934-368X ISO Abbreviation: Curr Protoc Immunol Publication Date: 2001 May |
Date Detail:
|
Created Date: 2008-04-24 Completed Date: 2008-09-29 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9101651 Medline TA: Curr Protoc Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: Unit 7.2 Citation Subset: IM |
Affiliation:
|
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Humans Rosette Formation / methods* T-Lymphocytes / cytology*, immunology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Measurement of cytotoxic activity of NK/LAK cells.
Next Document: Isolation and functional analysis of neutrophils.