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Isolation of Staphylococcus aureus from environmental surfaces in an academic dental clinic.
MedLine Citation:
PMID:  22298558     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: Staphylococcus aureus is an important health care-associated pathogen that often is resistant to antibiotics. The authors conducted a pilot study to determine if abiotic surfaces in a dental clinic were contaminated frequently.
METHODS: The authors sampled surfaces with swabs that they then used to inoculate selective and differential media. CHRO-Magar Staph aureus (DRG International, Mountainside, N.J.) was the most effective. They used phenotypic and genotypic tests to identify presumptive S. aureus colonies. They determined the sensitivity of S. aureus isolates to five antibiotics, including oxacillin, according to the Kirby-Bauer method.
RESULTS: The authors recovered S. aureus from 20 of 429 surfaces (4.7 percent). Most isolates were resistant to penicillin but none were resistant to the other antibiotics. No isolate carried the mecA gene encoding resistance to methicillin. The authors considered one site to be highly contaminated (> 200 colony-forming units [CFUs]), but all other sites that tested positive yielded fewer than 5 CFUs.
CONCLUSIONS: Abiotic surfaces in the authors' dental clinic were not a reservoir for methicillin-resistant S. aureus. The authors identified and eliminated one nonclinical site of potential methicillin-sensitive S. aureus cross-contamination.
CLINICAL IMPLICATIONS: Periodic sampling of surfaces for S. aureus may be a useful adjunct to standard infection control practices in dental health care settings.
Authors:
Denise A Trochesset; Stephen G Walker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the American Dental Association (1939)     Volume:  143     ISSN:  1943-4723     ISO Abbreviation:  J Am Dent Assoc     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7503060     Medline TA:  J Am Dent Assoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  164-9     Citation Subset:  D; IM    
Affiliation:
Dr. Trochesset is an assistant professor, Department of Oral Biology and Pathology, School of Dental Medicine, Stony Brook University, Stony Brook, N.Y.
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