| Isolation of Side Population Cells in B-Cell Non-Hodgkin's Lymphomas. | |
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MedLine Citation:
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PMID: 22964907 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: Side population (SP) cells are characterized by the ability to exclude Hoechst 33342 dye due to high expression of the ATP-binding cassette transporter. This ability is associated with drug-resistant characteristics of cancer stem cells. Methods: We analyzed SP cells from human B-cell non-Hodgkin's lymphoma cell lines and primary cells derived from patients and compared them with non-SP (NSP) cells. Results: SP cells comprised a minor fraction of all cells ranging from 1.5 ± 1.8 to 8.3 ± 5.7% in cell lines and had higher ABCG2 expression than NSP cells. SP cells had better cell viability, colony-forming ability and drug resistance than NSP cells. The SP cells also showed stem cell-like characteristics, including elevated telomerase activity and higher expression of OCT4 and NANOG. A cDNA microarray demonstrated that SP cells had decreased expression of genes associated with apoptosis and cell death compared to NSP cells. Conclusions: The presence of SP cells might imply the possibility of lymphoma stem cells and be associated with a malignant potential of B-cell lymphoma. |
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Authors:
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Mi Ran Lee; Hyun-Jeong Ju; Byung Soo Kim; Young Hyeh Ko; Won Seog Kim; Seok Jin Kim |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-08 |
Journal Detail:
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Title: Acta haematologica Volume: 129 ISSN: 1421-9662 ISO Abbreviation: Acta Haematol. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0141053 Medline TA: Acta Haematol Country: - |
Other Details:
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Languages: ENG Pagination: 10-17 Citation Subset: - |
Copyright Information:
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Copyright © 2012 S. Karger AG, Basel. |
Affiliation:
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Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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