Document Detail


Isolation of Side Population Cells in B-Cell Non-Hodgkin's Lymphomas.
MedLine Citation:
PMID:  22964907     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background: Side population (SP) cells are characterized by the ability to exclude Hoechst 33342 dye due to high expression of the ATP-binding cassette transporter. This ability is associated with drug-resistant characteristics of cancer stem cells. Methods: We analyzed SP cells from human B-cell non-Hodgkin's lymphoma cell lines and primary cells derived from patients and compared them with non-SP (NSP) cells. Results: SP cells comprised a minor fraction of all cells ranging from 1.5 ± 1.8 to 8.3 ± 5.7% in cell lines and had higher ABCG2 expression than NSP cells. SP cells had better cell viability, colony-forming ability and drug resistance than NSP cells. The SP cells also showed stem cell-like characteristics, including elevated telomerase activity and higher expression of OCT4 and NANOG. A cDNA microarray demonstrated that SP cells had decreased expression of genes associated with apoptosis and cell death compared to NSP cells. Conclusions: The presence of SP cells might imply the possibility of lymphoma stem cells and be associated with a malignant potential of B-cell lymphoma.
Authors:
Mi Ran Lee; Hyun-Jeong Ju; Byung Soo Kim; Young Hyeh Ko; Won Seog Kim; Seok Jin Kim
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-08
Journal Detail:
Title:  Acta haematologica     Volume:  129     ISSN:  1421-9662     ISO Abbreviation:  Acta Haematol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0141053     Medline TA:  Acta Haematol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  10-17     Citation Subset:  -    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Affiliation:
Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, Korea.
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