Document Detail

Isolation of mouse mammary epithelial subpopulations: a comparison of leading methods.
MedLine Citation:
PMID:  22644112     Owner:  NLM     Status:  MEDLINE    
Isolation of mammary epithelial subpopulations, including stem and progenitor cells, has become a standard technique in recent years. However, a number of methods and approaches for this have developed and the relative benefits of the different approaches, and the reason for their development, have not always been clear. Here, three of the leading laboratories working on the separation of mammary cell subpopulations have summarised their methods, highlighted their differences and similarities and also discussed the reasoning behind the approaches they have taken. This article will assist workers establishing mammary cell separation protocols in their laboratories to make informed choices about the methods they should use.
Matthew J Smalley; Howard Kendrick; Julie M Sheridan; Joseph L Regan; Michael D Prater; Geoffrey J Lindeman; Christine J Watson; Jane E Visvader; John Stingl
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2012-05-30
Journal Detail:
Title:  Journal of mammary gland biology and neoplasia     Volume:  17     ISSN:  1573-7039     ISO Abbreviation:  J Mammary Gland Biol Neoplasia     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-08-28     Completed Date:  2013-01-22     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9601804     Medline TA:  J Mammary Gland Biol Neoplasia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  91-7     Citation Subset:  IM    
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MeSH Terms
Adult Stem Cells / cytology*,  metabolism
Antibody Specificity
Antigens, Surface / metabolism
Cell Differentiation
Cell Separation / instrumentation,  methods*
Cells, Cultured
Epithelium / metabolism*
Mammary Glands, Animal / cytology*,  metabolism
Organoids / cytology,  metabolism
Staining and Labeling / methods
Grant Support
G0900980//Medical Research Council; //Cancer Research UK
Reg. No./Substance:
0/Antigens, Surface

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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