Document Detail

Isolation of human adrenocortical aldosterone-producing cells by a novel immunomagnetic beads method.
MedLine Citation:
PMID:  20097714     Owner:  NLM     Status:  MEDLINE    
We detected intense CD56 immunostaining in the zona glomerulosa (ZG) and medulla of the normal human adrenal gland and therefore identified CD56, the neural cell adhesion molecule, as a membrane antigen specific for the ZG, aldosterone-producing adenoma (APA), and chromaffin cells. The APA and pheochromocytoma cells, which are histogenetically derived from the ZG and medulla, respectively, also showed intense CD56 immunostaining. Based on these findings we developed a strategy for isolating cells from the ZG and APA using CD56 immunobinding to magnetic beads. Morphology, gene expression studies, and aldosterone measurement confirmed that CD56 positive (+) cells were ZG and APA cells. Analysis of CD56+ cells under light and phase contrast microscopy evidenced that these cells formed clumps, as the ZG cells usually do; with electron microscopy they showed multiple features typical of a steroidogenic phenotype. Expression levels of the CD56 and the aldosterone synthase (CYP11B2) gene were markedly higher in CD56+ cells than CD56- cells (+1600 and +2100% increase, respectively). Moreover, aldosterone secretion was higher (+1380%) from CD56+ cells than from CD56- cells. Hence, this novel methodology allows isolation of a pure population of ZG and APA cells exhibiting multiple characteristics of the aldosterone-producing cells.
Brasilina Caroccia; Ambrogio Fassina; Teresa Maria Seccia; Chiara Recarti; Lucia Petrelli; Anna Sandra Belloni; Maria Rosa Pelizzo; Gian Paolo Rossi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-22
Journal Detail:
Title:  Endocrinology     Volume:  151     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-03-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1375-80     Citation Subset:  AIM; IM    
Department of Clinical and Experimental Medicine, University of Padua, 35126 Padua, Italy.
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MeSH Terms
Adrenal Cortex Neoplasms / metabolism*
Adrenocortical Adenoma / metabolism*
Aldosterone / secretion*
Antigens, CD56 / metabolism*
Immunomagnetic Separation*
Zona Glomerulosa / cytology*,  metabolism
Reg. No./Substance:
0/Antigens, CD56; 52-39-1/Aldosterone

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