Document Detail

Isolation of Hokkaido virus, genus Hantavirus, using a newly established cell line derived from the kidney of the gray red-backed vole (Myodes rufocanus bedfordiae).
MedLine Citation:
PMID:  22791608     Owner:  NLM     Status:  Publisher    
Hantaviruses belong to the Bunyaviridae family and are maintained in wild rodents. Although VeroE6 cells, which originate from African green monkey kidney, are used widely in hantavirus research, isolation of hantaviruses from this cell line is difficult. To develop an efficient method of propagation and isolation of hantaviruses, we established a novel cell line, MRK101, derived from the kidney of the gray red-backed vole (Myodes rufocanus bedfordiae), the natural host of Hokkaido virus (HOKV). The MRK101 cells showed a significantly higher susceptibility to Puumala virus (PUUV) hosted by Myodes glareolus compared to VeroE6 cells. Viral nucleocapsid protein in PUUV-infected MRK101 cells was detected earlier than in VeroE6 cells, and the viral titer in the culture fluid of MRK101 cells was higher than that of VeroE6 cells during the early phase of infection. In contrast, MRK101 cells showed no susceptibility to Hantaan virus. HOKV, which has not been isolated to date, was successfully isolated using MRK101 cells. Moreover, the newly isolated HOKV was successfully propagated in MRK101, but not VeroE6, cells. Phylogenic analyses of the S, M, and L segment sequences revealed that HOKV is most closely related to PUUV, but is distinct from other hantaviruses. These data suggest that the MRK101 cell line is a useful tool for the isolation and propagation of hantaviruses. Moreover, this is the first report on hantavirus isolation in a cell line that originated from the natural host.
Takahiro Sanada; Takahiro Seto; Yuka Ozaki; Ngonda Saasa; Kumiko Yoshimatsu; Jiro Arikawa; Kentaro Yoshii; Hiroaki Kariwa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-12
Journal Detail:
Title:  The Journal of general virology     Volume:  -     ISSN:  1465-2099     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Hokkaido University.
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