Document Detail

Isolation of Helicobacter pylori from human faeces.
MedLine Citation:
PMID:  1359263     Owner:  NLM     Status:  MEDLINE    
Helicobacter pylori is arguably the commonest chronic infection in man. However, its route of transmission is unknown. We have isolated viable H pylori from the faeces of an infected individual from The Gambia. The organism was cultured on selective media after concentration of faecal bacteria by centrifugation in a buffer equilibrated with a microaerophilic gas mixture. Growth characteristics, microscopic appearances, and enzyme activities were the same as those of a typical gastric isolate of H pylori. Protein preparations derived from the new isolate and the typical strain were antigenically similar, and had very similar electrophoretic profiles (including two major protein bands of 62 and 26 kDa, corresponding to the urease enzyme subunits). With the same technique, organisms with the colony morphology, growth requirements, enzyme activities, and microscopic appearances of H pylori were isolated from the faeces of 9 of 23 randomly selected children aged 3-27 months from a Gambian village with a high prevalence of H pylori infection in early life. Faecal-oral transmission is probably important in the spread of infection in such communities.
J E Thomas; G R Gibson; M K Darboe; A Dale; L T Weaver
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lancet     Volume:  340     ISSN:  0140-6736     ISO Abbreviation:  Lancet     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1992-12-17     Completed Date:  1992-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985213R     Medline TA:  Lancet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1194-5     Citation Subset:  AIM; IM    
MRC Dunn Nutrition Centre, Cambridge, UK.
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MeSH Terms
Antigens, Bacterial / analysis
Child, Preschool
Electrophoresis, Polyacrylamide Gel
Feces / microbiology*
Helicobacter pylori / classification,  immunology,  isolation & purification*
Reg. No./Substance:
0/Antigens, Bacterial
Comment In:
Lancet. 1993 Feb 6;341(8841):380   [PMID:  8094153 ]
Lancet. 1993 Dec 4;342(8884):1419-20   [PMID:  7901696 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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