Document Detail


Isolation of HLA locus-specific DNA probes from the 3'-untranslated region.
MedLine Citation:
PMID:  6591185     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When human class I cDNA clones containing coding sequences are used to probe genomic DNA, 15-20 fragments, each containing a complete class I gene or pseudogene, are seen. Identification of which genomic DNA segments encode the HLA-A and -B antigens has to date required transfection of mouse L cells with cloned class I genes or analysis of HLA loss mutants. In this report we show that under high-stringency conditions, probes constructed from the 3'-untranslated region can be used to specifically identify the segments of DNA that encode the HLA-A and -B antigens in the human lymphoblastoid cell line 721. Examination of DNA from unrelated individuals indicates that these probes are locus specific and will permit identification of HLA-A and -B genes in the population.
Authors:
B H Koller; B Sidwell; R DeMars; H T Orr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  81     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1984 Aug 
Date Detail:
Created Date:  1984-10-03     Completed Date:  1984-10-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5175-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cloning, Molecular*
DNA / isolation & purification*
Genes*
HLA Antigens / genetics*
Humans
Leukemia, Lymphoid
Mutation
Protein Biosynthesis*
Grant Support
ID/Acronym/Agency:
AI-15486/AI/NIAID NIH HHS; AI-18124/AI/NIAID NIH HHS; P30 CA 14520/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/HLA Antigens; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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