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Isolation, Growth, and Characterization of Human Renal Epithelial Cells Using Traditional and 3D Methods.
MedLine Citation:
PMID:  23097116     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The kidney is a highly heterogeneous organ that is responsible for fluid and electrolyte balance. Much interest is focused on determining the function of specific renal epithelial cells in humans, which can only be accomplished through the isolation and growth of nephron segment-specific epithelial cells. However, human renal epithelial cells are notoriously difficult to maintain in culture. This chapter describes the isolation, growth, immortalization, and characterization of the human renal proximal tubule cell. In addition, we describe new paradigms in 3D cell culture which allow the cells to maintain more in vivo-like morphology and function.
Authors:
John J Gildea; Helen E McGrath; Robert E Van Sciver; Dora Bigler Wang; Robin A Felder
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  945     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  329-45     Citation Subset:  IM    
Affiliation:
Department of Pathology, The University of Virginia, Charlottesville, VA, USA.
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