Document Detail


Isolation of Delta12 and omega3-fatty acid desaturase genes from the yeast Kluyveromyces lactis and their heterologous expression to produce linoleic and alpha-linolenic acids in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  16823888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two clones with homology to known fatty acid desaturase genes were isolated from the yeast Kluyveromyces lactis. The first gene, which we designate KlFAD2, consists of 411 amino acids with an overall identity of 73.0% to FAD2 from Saccharomyces kluyveri. It exhibited Delta12 fatty acid desaturase activity when expressed in S. cerevisiae under the control of ADH1 promoter and produced endogenous linoleic acid. The second clone, which we designate KlFAD3, consists of 415 amino acids with an overall identity of 79.3% to FAD3 from S. kluyveri. It exhibited omega3 fatty acid desaturase activity in S. cerevisiae when expressed under the control of ADH1 promoter in the presence of the exogenous substrate linoleic acid and produced alpha-linolenic acid. Co-expression of KlFAD2 and KlFAD3 resulted in the endogenous production of both linoleic and alpha-linolenic acids. The yield of alpha-linolenic acid reached 0.8% of total fatty acids and its production was not increased by adding exogenous oleic acid; alpha-linolenic acid reached 8.7% when exogenous linoleic acid was available.
Authors:
Kumiko Kainou; Yasushi Kamisaka; Kazuyoshi Kimura; Hiroshi Uemura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  23     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-07-12     Completed Date:  2006-09-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  England    
Other Details:
Languages:  eng     Pagination:  605-12     Citation Subset:  IM    
Copyright Information:
Copyright 2006 John Wiley & Sons, Ltd.
Affiliation:
National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Chromatography, Gas
Cloning, Molecular
DNA, Fungal / chemistry,  genetics
Escherichia coli / genetics
Fatty Acid Desaturases / genetics*,  metabolism
Fatty Acids / analysis
Kluyveromyces / enzymology*,  genetics*,  metabolism
Linoleic Acid / biosynthesis*,  genetics
Molecular Sequence Data
Phylogeny
Plasmids / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Sequence Analysis, DNA
alpha-Linolenic Acid / biosynthesis*,  genetics
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/Fatty Acids; 2197-37-7/Linoleic Acid; 463-40-1/alpha-Linolenic Acid; EC 1.14.19.-/Fatty Acid Desaturases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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