| Isolation of the DLD gene of Saccharomyces cerevisiae encoding the mitochondrial enzyme D-lactate ferricytochrome c oxidoreductase. | |
| | |
MedLine Citation:
|
PMID: 8492799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In Saccharomyces cerevisiae the utilization of lactate occurs via specific oxidation of L- and D-lactate to pyruvate catalysed by L-lactate ferricytochrome c oxidoreductase (L-LCR) (EC 1.1.2.3) encoded by the CYB2 gene, and D-lactate ferricytochrome c oxidoreductase (D-LCR) (EC 1.1.2.4), respectively. We selected several lactate- pyruvate+ mutants in a cyb2 genetic background. Two of them were devoid of D-LCR activity (dld mutants, belonging to the same complementation group). The mutation mapped in the structural gene. This was demonstrated by a gene dosage effect and by the thermosensitivity of the enzyme activity of thermosensitive revertants. The DLD gene was cloned by complementation for growth on D-, L-lactate in the strain WWF18-3D, carrying both a CYB2 disruption and the dld mutation. The minimal complete complementing sequence was localized by subcloning experiments. From the sequence analysis an open reading frame (ORF) was identified that could encode a polypeptide of 576 amino-acids, corresponding to a calculated molecular weight of 64000 Da. The deduced protein sequence showed significant homology with the previously described microsomal flavoprotein L-gulono-gamma-lactone oxidase isolated from Rattus norvegicus, which catalyses the terminal step of L-ascorbic acid biosynthesis. These results are discussed together with the role of L-LCR and D-LCR in lactate metabolism of S. cerevisiae. |
| | |
Authors:
|
T Lodi; I Ferrero |
Related Documents
:
|
12035049 - Pcr based cloning and sequence analysis of the pichia pastoris cystathionine beta-synth... 8446029 - The general amino acid control regulates met4, which encodes a methionine-pathway-speci... 2017169 - The cdc25 protein of saccharomyces cerevisiae promotes exchange of guanine nucleotides ... 8890739 - Cloning and characterization of two yeast genes encoding members of the ccch class of z... 17435999 - Novel group vii histidine kinase hwhhk7b from the halophilic fungi hortaea werneckii ha... 8824839 - Molecular cloning of a genomic dna for enolase from aspergillus oryzae. 12083769 - Srebp-2 and nf-y are involved in the transcriptional regulation of squalene epoxidase. 8319909 - A small nucleolar rna is processed from an intron of the human gene encoding ribosomal ... 19383159 - Estpiper--a web-based analysis pipeline for expressed sequence tags. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Molecular & general genetics : MGG Volume: 238 ISSN: 0026-8925 ISO Abbreviation: Mol. Gen. Genet. Publication Date: 1993 Apr |
Date Detail:
|
Created Date: 1993-06-17 Completed Date: 1993-06-17 Revised Date: 2010-04-12 |
Medline Journal Info:
|
Nlm Unique ID: 0125036 Medline TA: Mol Gen Genet Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 315-24 Citation Subset: IM |
Affiliation:
|
Institute of Genetics, University of Parma, Italy. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/X66052 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cytochromes / biosynthesis Genes, Fungal* L-Lactate Dehydrogenase / genetics* Lactate Dehydrogenases* Mitochondria / enzymology Molecular Sequence Data Mutation Plasmids Rats Saccharomyces cerevisiae / enzymology*, genetics |
| Chemical | |
Reg. No./Substance:
|
0/Cytochromes; EC 1.1.-/Lactate Dehydrogenases; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.1.2.4/D-lactate dehydrogenase (cytochrome) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The sequence-specific nuclear matrix binding factor F6 is a chicken GATA-like protein.
Next Document: Transcript identification in the optomotor-blind locus of Drosophila melanogaster by intragenic reco...