Document Detail


Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.
MedLine Citation:
PMID:  6933469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mutants of Chinese hamster ovary cells lacking dihydrofolate reductase (tetrahydrofolate dehydrogenase, 7,8-dihydrofolate:NADP+ oxidoreductase; EC 1.5.1.3) activity were isolated after mutagenesis and exposure to high-specific-activity [3H]deoxyuridine as a selective agent. Fully deficient mutants could not be isolated starting with wild-type cells, but could readily be selected from a putative heterozygote that contains half of the wild-type level of dihydrofolate reductase activity. The heterozygote itself was selected from wild-type cells by using [3H]deoxyuridine together with methotrexate to reduce intracellular dihydrofolate reductase activity. Fully deficient mutants require glycine, a purine, and thymidine for growth; this phenotype is recessive to wild type in cell hybrids. Revertants have been isolated, one of which produces a heat-labile dihydrofolate reductase activity. These mutants may be useful for metabolic studies relating to cancer chemotherapy and for fine-structure genetic mapping of mutations by using available molecular probes for this gene.
Authors:
G Urlaub; L A Chasin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  77     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1980 Jul 
Date Detail:
Created Date:  1981-01-29     Completed Date:  1981-01-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4216-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line*
Cricetinae
Cricetulus
Female
Heterozygote
Hybrid Cells / enzymology
Mutation
Ovary
Selection, Genetic
Tetrahydrofolate Dehydrogenase / deficiency*,  genetics
Grant Support
ID/Acronym/Agency:
GM-22629/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
EC 1.5.1.3/Tetrahydrofolate Dehydrogenase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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