Document Detail

Isolation and characterization of spheroid cells from human malignant melanoma cell line WM-266-4.
MedLine Citation:
PMID:  19940551     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: Spheroid cells which can grow as nonattached spheroids in vitro culture condition are considered as tumor-initiating cells that have properties similar to those of stem cells. However, the existence of spheroid cells in WM-266-4, a human malignant metastatic melanoma cell line, has not yet been reported. METHODS: Accordingly, we investigated whether WM-266-4 can form spheroids, and characterized these spheroids using qRT-PCR, histology, immunohistochemistry and xenograft. RESULTS: WM-266-4 contains a small subpopulation of cells that grow as spheroids and express genes strongly related to tumor malignancy and stem-like factors. Second, histological analysis of the spheres revealed that they consist of 300-400 round cells per sphere with a high karyoplasmic ratio. They have a basophilic cytoplasm and are highly pleomorphic in size, and sometimes multinucleated and giant. Third, although there were differences between the spheroid and bulk cells, they both have high tumorigenic potential, as both cell types formed a tumor mass upon injection of only 100 cells in nude mice. CONCLUSION: We characterized the spheroid cells in an established melanoma cell line. We suggest that enriched spheroid cells might contain more dedifferentiated progenitor cells, but we could not conclude spheroid cells are cancer stem cells.
Y R Na; S H Seok; D J Kim; J H Han; T H Kim; H Jung; B H Lee; J H Park
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-23
Journal Detail:
Title:  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine     Volume:  30     ISSN:  1423-0380     ISO Abbreviation:  Tumour Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2010-01-06     Completed Date:  2010-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8409922     Medline TA:  Tumour Biol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  300-9     Citation Subset:  IM    
Copyright Information:
(c) 2009 S. Karger AG, Basel.
Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, Seoul, Republic of Korea.
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MeSH Terms
ATP-Binding Cassette Transporters / genetics
Antigens, CD / genetics
Antigens, Neoplasm / analysis
Cell Line, Tumor
Cell Proliferation
Gene Expression Regulation, Neoplastic
Glycoproteins / genetics
Homeodomain Proteins / genetics
Intermediate Filament Proteins / analysis,  genetics
Melanoma / genetics,  metabolism,  pathology*
Melanoma, Experimental / genetics,  metabolism,  pathology*
Mice, Nude
Neoplasm Proteins / analysis,  genetics
Nerve Tissue Proteins / analysis,  genetics
Nuclear Proteins / genetics
Peptides / genetics
Proto-Oncogene Proteins / genetics
Repressor Proteins / genetics
Reverse Transcriptase Polymerase Chain Reaction
Spheroids, Cellular / metabolism,  pathology*
Time Factors
Transplantation, Heterologous
Tumor Burden
Tumor Markers, Biological / analysis
Tumor Suppressor Proteins / genetics
Vascular Endothelial Growth Factor Receptor-3 / genetics
Wnt Proteins / genetics
Reg. No./Substance:
0/ABCG2 protein, human; 0/AC133 antigen; 0/ATP-Binding Cassette Transporters; 0/Antigens, CD; 0/Antigens, Neoplasm; 0/Glycoproteins; 0/Homeodomain Proteins; 0/Intermediate Filament Proteins; 0/MART1 antigen; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Nuclear Proteins; 0/Peptides; 0/Proto-Oncogene Proteins; 0/Repressor Proteins; 0/Tumor Markers, Biological; 0/Tumor Suppressor Proteins; 0/WNT5A protein, human; 0/Wnt Proteins; 0/nestin; 0/prospero-related homeobox 1 protein; 138791-04-5/BMI1 protein, human; EC Endothelial Growth Factor Receptor-3

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