Document Detail


Isolation and Characterization of a Neurotrophic Factor-like Substance from Venom of Agkistrodon acutus.
MedLine Citation:
PMID:  12136218     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A neurotrophic factor-like substance from Agkistrodon acutus was isolated by ion exchange and gel filtration chromatography and was found to be homogenous by electrophoresis. Itsmolecular weight was estimated to be approximately 26 kD by gel filtration. A characteristic of the substance was that the protein consisted of two subunits which were bound one to another noncovalently. The analytical isoelectric focusing revealed its isoelectric point of 7.8. The biological activity of the substance was comparable to that of mouse 2.5 S NGF. It rescued PC12 cells from apoptosis at the concentration interval of 1 100 &mgr;g/L and could induce PC12 cells differentiate to sympathetic-like neurons.
Authors:
Li-Ying Guo; Sha Pang; Qiao-Qiao Ruan; Yuan-Cong Zhou
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica     Volume:  31     ISSN:  0582-9879     ISO Abbreviation:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao     Publication Date:  1999  
Date Detail:
Created Date:  2002-Jul-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  20730160R     Medline TA:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  211-214     Citation Subset:  -    
Affiliation:
Shanghai Institute of Biochemistry the Chinese Academy of Sciences Shanghai 200031 China. zhouyc@sunm.shcnc.ac.cn
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