Document Detail


Isolation and Characterisation of Lytic Bacteriophages of Klebsiella pneumoniae and Klebsiella oxytoca.
MedLine Citation:
PMID:  23143289     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Klebsiella bacteria have emerged as an increasingly important cause of community-acquired nosocomial infections. Extensive use of broad-spectrum antibiotics in hospitalised patients has led to both increased carriage of Klebsiella and the development of multidrug-resistant strains that frequently produce extended-spectrum β-lactamases and/or other defences against antibiotics. Many of these strains are highly virulent and exhibit a strong propensity to spread. In this study, six lytic Klebsiella bacteriophages were isolated from sewage-contaminated river water in Georgia and characterised as phage therapy candidates. Two of the phages were investigated in greater detail. Biological properties, including phage morphology, nucleic acid composition, host range, growth phenotype, and thermal and pH stability were studied for all six phages. Limited sample sequencing was performed to define the phylogeny of the K. pneumoniae- and K. oxytoca-specific bacteriophages vB_Klp_5 and vB_Klox_2, respectively. Both of the latter phages had large burst sizes, efficient rates of adsorption and were stable under different adverse conditions. Phages reported in this study are double-stranded DNA bacterial viruses belonging to the families Podoviridae and Siphoviridae. One or more of the six phages was capable of efficiently lysing ~63 % of Klebsiella strains comprising a collection of 123 clinical isolates from Georgia and the United Kingdom. These phages exhibit a number of properties indicative of potential utility in phage therapy cocktails.
Authors:
Natia Karumidze; Ia Kusradze; Sophio Rigvava; Marine Goderdzishvili; Kumar Rajakumar; Zemphira Alavidze
Related Documents :
24170119 - A simple assay for measuring catalase activity: a visual approach.
24627729 - Essential oils, a new horizon in combating bacterial antibiotic resistance.
24480099 - Aseptic meningitis outbreak caused by echovirus 30 in two regions in bulgaria, may-augu...
23853369 - Bacteria growth monitoring through a differential cmos capacitive sensor.
23661459 - Genetic characterization of fusarium graminearum and f. culmorum isolates from turkey b...
23308379 - Genetic diversity of the ftsi gene in β-lactamase-nonproducing ampicillin-resistant an...
9096179 - Sensitivity testing of ciprofloxacin for pseudomonas aeruginosa.
22285899 - Plasmid-mediate transfer of flo1 into industrial saccharomyces cerevisiae pe-2 strain c...
24503209 - Heterologous production of spectinomycin in streptomyces venezuelae by exploiting the d...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-11
Journal Detail:
Title:  Current microbiology     Volume:  -     ISSN:  1432-0991     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilisi, Georgia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Isolation and Characterization of Antagonistic Bacillus Strains Capable to Degrade Ethylenethiourea.
Next Document:  Subjective and objective results of arthroscopic debridement of ulnar-sided TFCC (Palmer type 1B) le...