| Isolation of Burkholderia cepacia complex genomovars from waters. | |
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MedLine Citation:
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PMID: 14666988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to develop a selective enrichment broth as an aid for the isolation of Burkholderia cepacia complex (Bcc) bacteria from water. To allow growth of all nine genomovars, mixtures of two carbon sources had to be used, i.e. L-arabinose/D-cellobiose or L-arabinose/L-threonine. Selectivity was provided by polymyxin B and 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan (C-390). Following enrichment, Bcc bacteria were isolated on a diagnostic O/F agar supplemented with gentamicin. A preliminary bio-diversity study on 28 surface waters yielded five different genomovars, i.e. B. cepacia (genomovar I), B. multivorans, B. cenocepacia, B. vietnamiensis and B. anthina. Drinking waters did not contain Bcc bacteria. However, the genomovar pattern from a given sample varied with the enrichment broth used. |
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Authors:
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Karen Vermis; Mariya Brachkova; Peter Vandamme; Hans Nelis |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Systematic and applied microbiology Volume: 26 ISSN: 0723-2020 ISO Abbreviation: Syst. Appl. Microbiol. Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-12-11 Completed Date: 2004-02-04 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8306133 Medline TA: Syst Appl Microbiol Country: Germany |
Other Details:
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Languages: eng Pagination: 595-600 Citation Subset: IM |
Affiliation:
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Laboratory for Pharmaceutical Microbiology, University of Ghent, Ghent, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acridines
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pharmacology Anti-Bacterial Agents / pharmacology Arabinose / metabolism Burkholderia cepacia complex / drug effects, growth & development*, isolation & purification*, metabolism Cellobiose / metabolism Culture Media / chemistry DNA Fingerprinting DNA, Bacterial / isolation & purification Gentamicins / pharmacology Interspersed Repetitive Sequences / genetics Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Polymyxin B / pharmacology Rec A Recombinases / genetics Selection, Genetic Threonine / metabolism Water Microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Acridines; 0/Anti-Bacterial Agents; 0/Culture Media; 0/DNA, Bacterial; 0/Gentamicins; 1404-26-8/Polymyxin B; 147-81-9/Arabinose; 16462-44-5/Cellobiose; 72-19-5/Threonine; 77769-31-4/9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan; EC 2.7.7.-/Rec A Recombinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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