Document Detail

Isolation of a 3-deoxy-D-mannooctulosonic acid disaccharide from Salmonella minnesota rough-form lipopolysaccharides.
MedLine Citation:
PMID:  6682036     Owner:  NLM     Status:  MEDLINE    
Lipopolysaccharides of Salmonella minnesota rough mutants were treated with 20 mM acetate buffer pH 4.4 at 70 degrees C/3 h. After dialysis of the hydrolysates about one third of the total 3-deoxy-D-mannooctulosonic acid (dOclA) content but no neutral sugars were found in the dialysate. By high-voltage paper electrophoresis, a compound with the mobility of 1.2 relative to dOclA could be isolated from the dialysate. It was identified as a dOclA disaccharide by hydrolysis without or after reduction with sodium borohydride and by analysis with the thiobarbituric acid assay under different conditions. The ketosidic linkage in the disaccharide is assumed to be 2.4 or 2.5.
H Brade; C Galanos; O Lüderitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  131     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  201-3     Citation Subset:  IM    
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MeSH Terms
Disaccharides / isolation & purification*
Electrophoresis, Paper
Lipopolysaccharides / analysis*
Salmonella / analysis*,  genetics
Sugar Acids / isolation & purification*
Reg. No./Substance:
0/Disaccharides; 0/Lipopolysaccharides; 0/Sugar Acids; 1069-03-0/2-keto-3-deoxyoctonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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