Document Detail


Isolated microvesicles from peripheral blood and body fluids as observed by scanning electron microscope.
MedLine Citation:
PMID:  20199878     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microvesicles are sub-micron structures shed from the cell membrane in a final step of the budding process. After being released into the microenvironment they are free to move and carry signaling molecules to distant cells, thereby they represent a communication system within the body. Since all cells shed microvesicles, it can be expected that they will be found in different body fluids. The potential diagnostic value of microvesicles has been suggested, however, a standardized protocol for isolation has not yet been agreed upon. It is unclear what is the content of the isolates and whether the isolated microvesicles were present in vivo or-have they been created within the isolation procedure. To present evidence in this direction, in this work we focus on the visualization of the material obtained by the microvesicle isolation procedure. We present scanning electronic microscope images of microvesicles isolated from blood, ascites, pleural fluid, cerebrospinal fluid, postoperative drainage fluid and chyloid fluid acquired from human and animal patients. Vesicular structures sized from 1microm downto 50nm are present in isolates of all considered body fluids, however, the populations differ in size and shape reflecting also the composition of the corresponding sediments. Isolates of microvesicles contain numerous cells which indicates that methods of isolation and determination of the number of microvesicles in the peripheral blood are to be elaborated and improved.
Authors:
Anita Mrvar-Brecko; Vid Sustar; Vid Jansa; Roman Stukelj; Rado Jansa; Emir Mujagić; Peter Kruljc; Ales Iglic; Henry Hägerstrand; Veronika Kralj-Iglic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-03
Journal Detail:
Title:  Blood cells, molecules & diseases     Volume:  44     ISSN:  1096-0961     ISO Abbreviation:  Blood Cells Mol. Dis.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-29     Completed Date:  2010-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9509932     Medline TA:  Blood Cells Mol Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  307-12     Citation Subset:  IM    
Affiliation:
Laboratory of Clinical Biophysics, Faculty of Medicine, University of Ljubljana, Slovenia; University Medical Center Ljubljana, Slovenia.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / complications,  veterinary
Aged
Animals
Ascites / etiology,  pathology
Blood*
Body Fluids*
Carcinoma / blood,  complications
Cat Diseases / pathology
Cats
Cell-Derived Microparticles / ultrastructure*
Chylothorax / pathology,  veterinary
Colonic Neoplasms / complications
Female
Horses / blood
Humans
Lung Neoplasms / complications,  veterinary
Microscopy, Electron, Scanning*
Pancreatic Neoplasms / blood
Particle Size
Peritonitis / complications
Pleural Effusion, Malignant / etiology,  pathology
Subarachnoid Hemorrhage / cerebrospinal fluid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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