| Isolated canine and murine intestinal cells exhibit a different pattern of fuel utilization for oxidative metabolism. | |
| | |
MedLine Citation:
|
PMID: 12019609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The amount and type of dietary fiber influences the end-products of fermentation and thus fuel availability to intestinal tissue. Metabolic fuel usage was studied in intestinal cells isolated from dogs consuming a commercial diet or from rats consuming either a commercial rat diet or dog diet to examine preferential fuel usage, the effect of diet, and species differences. Production of 14CO2 was measured by incubating cells in media containing either D-[U-14C]glucose, [1-14C]n-butyrate, L-[U-14C]glutamine, or [1-14C]propionate with or without competing substrates. The presence of a mixture of 5 mM each of glucose, butyrate, propionate, and acetate and 1 mM glutamine in the media decreased CO2 production from glucose, glutamine, and propionate by canine enterocytes (P < 0.05) and from glutamine and propionate by canine colonocytes (P < 0.05). The presence of glutamine in the media decreased glucose oxidation by murine enterocytes, regardless of the diet. Similarly, glutamine decreased glucose oxidation by murine colonocytes (P < 0.05), but only when the rats had consumed the rat diet. Regardless of diet, murine colonocytes oxidized more butyrate (P < 0.01) than did enterocytes, and murine enterocytes tended (P < 0.07) to oxidize more glucose than did colonocytes. The proportion of propionate in colonic contents was higher in dogs than in rats (P < 0.02), and the proportion of butyrate tended to be higher in contents from rats than in those from dogs (P < 0.08). Colonic and cecal wet weights were decreased (P < 0.05) when rats were fed the commercial dog diet. Preferred utilization of substrates by isolated canine enterocytes and colonocytes differed from that of murine intestinal cells. These differences were only partially overcome by feeding the same diet to each species. |
| | |
Authors:
|
A D Beaulieu; J K Drackley; T R Overton; L S Emmert |
Related Documents
:
|
3341249 - Intestinal absorption of dietary fat from a liquid diet perfused in rats at a submaximu... 3440339 - Isocal and portagen alter intestinal uptake of sugars but not mucosal surface area in r... 12479649 - Potato glycoalkaloids adversely affect intestinal permeability and aggravate inflammato... 16365069 - Dietary inulin intake and age can affect intestinal absorption of zinc and copper in rats. 15539269 - Effects of dietary polyamines and clofibrate on metabolism of polyamines in the rat. 19330069 - Intestinal structure and function of broiler chickens on diets supplemented with a synb... 15107599 - Comparison of cholesterol-lowering efficacy and anti-atherogenic properties of hydrogen... 9690629 - Influence of dietary magnesium level on growth-performance and metabolic responses of h... 20157589 - Fat accumulation in caenorhabditis elegans triggered by the electrophilic lipid peroxid... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of animal science Volume: 80 ISSN: 0021-8812 ISO Abbreviation: J. Anim. Sci. Publication Date: 2002 May |
Date Detail:
|
Created Date: 2002-05-21 Completed Date: 2002-10-02 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 1223-32 Citation Subset: IM |
Affiliation:
|
Department of Animal Sciences, University of Illinois, Urbana 61801, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetates
/
metabolism Animals Butyric Acids / metabolism Carbon Dioxide / metabolism Carbon Isotopes Cells, Cultured Colon / cytology*, metabolism* Diet / veterinary* Dietary Fiber / metabolism Dogs Enterocytes / metabolism Fermentation Glucose / metabolism Glutamine / metabolism Intestine, Small / cytology*, metabolism* Male Models, Animal Oxidation-Reduction Oxygen Consumption* Propionates / metabolism Rats Rats, Sprague-Dawley Species Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Acetates; 0/Butyric Acids; 0/Carbon Isotopes; 0/Propionates; 124-38-9/Carbon Dioxide; 50-99-7/Glucose; 56-85-9/Glutamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: National Beef Quality Audit-2000: survey of targeted cattle and carcass characteristics related to q...
Next Document: Changes in oxygen content and acid-base balance in arterial and portal blood in response to the diet...