| Isolated Kupffer cells, endothelial cells and hepatocytes as investigative tools for liver research. | |
| | |
MedLine Citation:
|
PMID: 7021184 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The preparation and utilization of purified liver cell subpopulation is becoming an established tool for laboratory research in biological sciences. The various methods employed utilize cell separation based on differential sensitivity to enzymatic digestion, differences in cell size-density characteristics, and differential adherence of the various cell populations to a glass or plastic substrate. As detailed here, the choice of a technique for cell isolation is determined not only by whether parenchymal or nonparenchymal populations are to be used, but also by considerations relevant for the study of specific physiologic parameters. In this paper, techniques for the isolation and enrichment of hepatocytes and Kupffer cells are reviewed, with special attention to the utilization of counterflow elutriation in the preparation of these and other purified nonparenchymal cell populations. As examples of how isolated cells provide useful insights, studies on the properties of hepatocyte and nonparenchymal liver cell glycoprotein receptors, as well as observations on the immunologic functioned capabilities of isolated Kupffer cells, are presented. |
| | |
Authors:
|
R N Zahlten; T M Rogoff; C J Steer |
Related Documents
:
|
3283454 - The role of intestinal flora on the interactions between nonparenchymal cells and hepat... 395294 - Exoerythrocytic merozoites of plasmodium berghei in rat hepatic kupffer cells. 8138244 - Stimulation of phagocytic activity of murine kupffer cells by tuftsin. 18853744 - Degradation of plectin with modulation of cytokeratin 18 in human liver cells during st... 7623844 - Activated h-ras rescues e1a-induced apoptosis and cooperates with e1a to overcome p53-d... 3236054 - Convergence of retinal w-cell and corticotectal input to cells of the cat superior coll... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.; Review |
Journal Detail:
|
Title: Federation proceedings Volume: 40 ISSN: 0014-9446 ISO Abbreviation: Fed. Proc. Publication Date: 1981 Aug |
Date Detail:
|
Created Date: 1981-10-14 Completed Date: 1981-10-14 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0372771 Medline TA: Fed Proc Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 2460-8 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Separation / methods Endothelium / cytology, metabolism Humans Kupffer Cells / immunology, metabolism, ultrastructure Liver / cytology*, metabolism, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
|
5PO1AM19329/AM/NIADDK NIH HHS; 5T32AM07100/AM/NIADDK NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Contraceptive activity and mechanism of action of combinations of 6-methyl-substituted D'6-pentarane...
Next Document: Primary hepatocyte culture: substratum requirements and production of matrix components.