Document Detail

Isogloboside biosynthesis in metastatic R3230AC cells results from a decreased GM3 synthase activity.
MedLine Citation:
PMID:  11339807     Owner:  NLM     Status:  MEDLINE    
We have determined that the production of a metastasis-associated neutral glycosphingolipid, isogloboside (iGb(4)Cer, GalNAcbeta1-3Galalpha1-3Galbeta1-4Glcbeta1-O-ceramide) is associated with the loss of G(M3) synthase activity. Assays for neutral glycosphingolipid-forming glycosyltransferases in cells producing various levels of iGb(4)Cer revealed no consistent differences that could account for the difference in iGb(4)Cer biosynthesis. However, comparison of the activity of G(M3) synthase in homogenates of these two cell types revealed that cells that did not synthesize iGb(4)Cer had activity significantly greater than that of cells possessing this antigen. Furthermore, somatic cell hybrids generated using clones of the iGb(4)Cer -producing and nonproducing cell lines lacked iGb(4)Cer while possessing high levels of G(M3) synthase activity. When iGb(4)Cer-producing cells were transfected with a G(M3) synthase expression vector, all of the resultant clones were negative for iGb(4)Cer production. The results of these studies clearly show that the presence of G(M3) synthase prevents the formation of iGb(4)Cer in these cells.
T Dumonceaux; S A Carlsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  389     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-07     Completed Date:  2001-06-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-94     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Microbiology, College of Medicine, Cancer Research Unit, University of Saskatchewan, Saskatchewan Cancer Agency, 20 Campus Drive, Saskatoon, Saskatchewan, Canada S7N 4H4.
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MeSH Terms
Carbohydrate Sequence
DNA Primers / genetics
Globosides / biosynthesis*,  chemistry
Hybrid Cells
Mammary Neoplasms, Experimental / enzymology,  genetics,  metabolism*,  secondary
Molecular Sequence Data
Sialyltransferases / deficiency*,  genetics
Tumor Cells, Cultured
Reg. No./Substance:
0/DNA Primers; 0/Globosides; 12244-28-9/cytolipin R; EC 2.4.99.-/Sialyltransferases; EC synthetase

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