| Isoforms and functions of NAD(P)H oxidase at the macula densa. | |
| | |
MedLine Citation:
|
PMID: 19204183 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Macula densa cells produce superoxide (O2-) during tubuloglomerular feedback primarily via NAD(P)H oxidase (NOX). The purpose of the present study was to determine NOXs expressed by the macula densa and the role of each one in NaCl-induced O2- production. To identify which isoforms are expressed, we applied single-cell RT-PCR to macula densa cells isolated by laser capture microdissection and to MMDD1 cells (a macula densa-like cell line). The captured cells expressed neuronal NOS (marker of macula densa), NOX2, and NOX4 but not NOX1. Expression of the NOXs and neuronal NOS was essentially identical in the MMDD1 cells. Thus, we used MMDD1 cells to investigate which isoform is responsible for NaCl-induced O2- production. We used small-interfering RNA to knock down NOX2 or NOX4 in MMDD1 cells and measured O2- exposed to low-salt solution (LS; 70 mmol/L of NaCl) or high-salt solution (HS; 140 mmol/L of NaCl). Exposing control cells (scrambled small-interfering RNA) to HS increased O2- concentrations from 0.75+/-0.28 to 1.48+/-0.46 U/min per 10(5) cells in LS and HS, respectively (P<0.001). Inhibiting NOX2 blocked the HS-induced increase in O2- (0.62+/-0.39 versus 0.76+/-0.31 U/min per 10(5) cells in LS and HS groups, respectively). Blocking NOX4 did not affect HS-induced O2- levels. O2- levels in the control cells during LS and HS were 0.80+/-0.30 and 1.56+/-0.49 U/min per 10(5) cells, respectively (P<0.001); whereas O2- levels in NOX4-small-interfering RNA-treated cells during LS and HS were 0.40+/-0.25 and 1.26+/-0.51 U/min per 10(5) cells, respectively (P<0.001). We conclude that, whereas macula densa cells express the NOX2 and NOX4 isoforms, NOX2 is primarily responsible for NaCl-induced O2- generation. |
| | |
Authors:
|
Rui Zhang; Pamela Harding; Jeffrey L Garvin; Ramiro Juncos; Ed Peterson; Luis A Juncos; Ruisheng Liu |
Related Documents
:
|
18845543 - Mechanical induction of an epithelial cell chymase associated with wound edge migration. 3236003 - Resonance raman spectroscopy of hemoglobin in intact cells: a probe of oxygen uptake by... 21947243 - Receptor for advanced glycation end products (rage) partially mediates hmgb1-erks activ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-02-09 |
Journal Detail:
|
Title: Hypertension Volume: 53 ISSN: 1524-4563 ISO Abbreviation: Hypertension Publication Date: 2009 Mar |
Date Detail:
|
Created Date: 2009-02-20 Completed Date: 2009-03-09 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 7906255 Medline TA: Hypertension Country: United States |
Other Details:
|
Languages: eng Pagination: 556-63 Citation Subset: IM |
Affiliation:
|
Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39216, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine
/
physiology Animals Cell Line Epithelial Cells / cytology, drug effects, metabolism Feedback, Physiological / physiology* Isoenzymes / metabolism Kidney Glomerulus / blood supply, drug effects, metabolism* Male Membrane Glycoproteins / metabolism* Mice Microcirculation / drug effects, physiology NADPH Oxidase / metabolism* Oxygen / metabolism Rats Rats, Sprague-Dawley Sodium Chloride / pharmacology Tumor Necrosis Factor-alpha / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
R01 DK073401-04/DK/NIDDK NIH HHS; R01 HL086767-02/HL/NHLBI NIH HHS; R01-DK073401/DK/NIDDK NIH HHS; R01-HL086767/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Isoenzymes; 0/Membrane Glycoproteins; 0/Tumor Necrosis Factor-alpha; 58-61-7/Adenosine; 7647-14-5/Sodium Chloride; 7782-44-7/Oxygen; EC 1.6.-/Cybb protein, rat; EC 1.6.-/Nox4 protein, mouse; EC 1.6.-/Nox4 protein, rat; EC 1.6.3.1/Cybb protein, mouse; EC 1.6.3.1/NADPH Oxidase |
| Comments/Corrections | |
Comment In:
|
Hypertension. 2009 Mar;53(3):456-7
[PMID:
19204181
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immediate and sustained blood pressure lowering by urocortin 2: a novel approach to antihypertensive...
Next Document: Targeted expression of receptor-associated late transducer inhibits maladaptive hypertrophy via bloc...