Document Detail


Isoforms of cellular fibronectin and tenascin in amniotic fluid.
MedLine Citation:
PMID:  7507066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amniotic fluid (AF) obtained from second trimester pregnancies presented extradomain (ED) A, B and an oncofetal (onc-f) domain containing isoforms of cellular fibronectin (cFn) in Western blotting of gelatin-bound polypeptides and directly of AF. Western blotting after sequential immunoprecipitation suggested at least three Fn molecules: one containing EDA and the onc-f domain and another minor component distinctly containing all the domains, and a third one only containing EDA. The immunoblotting results for EDA-cFn and onc-f-cFn were closely similar to that for total Fn, whereas in plasma samples of normal and pregnant women only traces of EDA-cFn and onc-f-cFn, but no EDB-cFn, were found. Western blotting of AF also indicated the presence of three isoforms of tenascin (Tn), M(r) 190,000 and 280,000 polypeptides earlier found in many cells, and a M(r) 200,000 polypeptide, novel for AF and not present in plasma. The results suggest a novel extracellular matrix polypeptide composition for AF.
Authors:
A Linnala; H von Koskull; I Virtanen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  337     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-02-18     Completed Date:  1994-02-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  167-70     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Amniocentesis
Amniotic Fluid / chemistry*
Antibodies, Monoclonal
Blotting, Western
Cell Adhesion Molecules, Neuronal / analysis*,  blood,  isolation & purification
Electrophoresis, Polyacrylamide Gel
Extracellular Matrix Proteins / analysis*,  blood,  isolation & purification
Female
Fibronectins / analysis*,  blood,  isolation & purification
Humans
Pregnancy / blood,  physiology*
Reference Values
Tenascin
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Cell Adhesion Molecules, Neuronal; 0/Extracellular Matrix Proteins; 0/Fibronectins; 0/Tenascin

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