Document Detail

Isoform 111 of Vascular Endothelial Growth Factor (VEGF111) Improves Angiogenesis of Ovarian Tissue Xenotransplantation.
MedLine Citation:
PMID:  23380862     Owner:  NLM     Status:  Publisher    
BACKGROUND: Cryopreservation of cortex ovarian tissue before anticancer therapy is a promising technique for fertility preservation mainly in children and young women. Ischemia in the early stage after ovarian graft causes massive follicle loss by apoptosis. VEGF111 is a recently described vascular endothelial growth factor (VEGF) isoform that does not bind to the extracellular matrix, diffuses extensively, and is resistant to proteolysis. These properties confer a significantly higher angiogenic potential to VEGF111 in comparison with the other VEGF isoforms. METHODS: We evaluated the morphology of cryopreserved sheep ovarian cortex grafted in the presence or absence of VEGF111. Ovarian cortex biopsies were embedded in type I collagen with or without VEGF111 addition before transplantation to severe combined immunodeficient mice ovaries. Transplants were retrieved 3 days or 3 weeks later. Follicular density, vasculature network, hemoglobin content, and cell proliferation were analyzed. RESULTS: Addition of VEGF111 increased density of functional capillaries (P=0.01) 3 days after grafting. By double immunostaining of Ki-67 and von Willebrand factor, we demonstrated that proliferating endothelial cells were found in 83% of the VEGF111 group compared with 33% in the control group (P=0.001). This angiostimulation was associated with a significant enhancement of hemoglobin content (P=0.03). Three weeks after transplantation, the number of primary follicles was significantly higher in VEGF111 grafts (P=0.02). CONCLUSION: VEGF111 accelerates blood vessel recruitment and functional angiogenesis and improves the viability of ovarian cortex by limiting ischemia and ovarian cortex damage.
Soraya Labied; Yves Delforge; Carine Munaut; Silvia Blacher; Alain Colige; Romain Delcombel; Laurie Henry; Maïté Fransolet; Caroline Jouan; Sophie Perrier d'Hauterive; Agnès Noël; Michelle Nisolle; Jean-Michel Foidart
Publication Detail:
Journal Detail:
Title:  Transplantation     Volume:  95     ISSN:  1534-6080     ISO Abbreviation:  Transplantation     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  -    
Other Details:
Languages:  ENG     Pagination:  426-433     Citation Subset:  -    
1 Laboratory of Tumor and Development Biology, Groupe Interdisciplinaire de Génoprotéomique Appliqué (GIGA-R), Université de Liège, Liège, Belgium. 2 Laboratory of Connective Tissues Biology, Groupe Interdisciplinaire de Génoprotéomique Appliqué (GIGA-R), Université de Liège, Liège, Belgium. 3 Department of Gynecology, Université de Liège, Liège, Belgium. 4 The CPMA Department, Université de Liège, Liège, Belgium. 5 Address correspondence to: Soraya Labied, Ph.D., Laboratory of Tumor and Development Biology, Université de Liège, Tour de Pathologie (B23), Sart-Tilman, B-4000 Liège, Belgium.
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