Document Detail

Isoflurane-induced acidosis depresses basal and PGE(2)-stimulated duodenal bicarbonate secretion in mice.
MedLine Citation:
PMID:  17158257     Owner:  NLM     Status:  MEDLINE    
When running in vivo experiments, it is imperative to keep arterial blood pressure and acid-base parameters within the normal physiological range. The aim of this investigation was to explore the consequences of anesthesia-induced acidosis on basal and PGE(2)-stimulated duodenal bicarbonate secretion. Mice (strain C57bl/6J) were kept anesthetized by a spontaneous inhalation of isoflurane. Mean arterial blood pressure (MAP), arterial acid-base balance, and duodenal mucosal bicarbonate secretion (DMBS) were studied. Two intra-arterial fluid support strategies were used: a standard Ringer solution and an isotonic Na(2)CO(3) solution. Duodenal single perfusion was used, and DMBS was assessed by back titration of the effluent. PGE(2) was used to stimulate DMBS. In Ringer solution-infused mice, isoflurane-induced acidosis became worse with time. The blood pH was 7.15-7.21 and the base excess was about -8 mM at the end of experiments. The continuous infusion of Na(2)CO(3) solution completely compensated for the acidosis. The blood pH was 7.36-7.37 and base excess was about 1 mM at the end of the experiment. Basal and PGE(2)-stimulated DMBS were markedly greater in animals treated with Na(2)CO(3) solution than in those treated with Ringer solution. MAP was slightly higher after Na(2)CO(3) solution infusion than after Ringer solution infusion. We concluded that isoflurane-induced acidosis markedly depresses basal and PGE(2)-stimulated DMBS as well as the responsiveness to PGE(2), effects prevented by a continuous infusion of Na(2)CO(3). When performing in vivo experiments in isoflurane-anesthetized mice, it is recommended to supplement with a Na(2)CO(3) infusion to maintain a normal acid-base balance.
Markus Sjöblom; Olof Nylander
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-07
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  292     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-09     Completed Date:  2007-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G899-904     Citation Subset:  IM    
Division of Physiology, Dept. of Neuroscience, Uppsala University, BMC, PO Box 572, Uppsala SE-751 23, Sweden.
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MeSH Terms
Acid-Base Equilibrium / drug effects
Acidosis / blood,  chemically induced,  metabolism*
Bicarbonates / metabolism*
Blood Pressure / drug effects
Carbon Dioxide / blood
Carbonates / blood,  pharmacology
Dinoprostone / pharmacology*
Duodenum / drug effects*,  secretion
Hydrogen-Ion Concentration / drug effects
Intestinal Mucosa / drug effects,  secretion
Mice, Inbred C57BL
Partial Pressure
Reg. No./Substance:
0/Bicarbonates; 0/Carbonates; 124-38-9/Carbon Dioxide; 26675-46-7/Isoflurane; 363-24-6/Dinoprostone; 497-19-8/sodium carbonate

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