Document Detail


Isoflurane attenuates dynorphin-induced cytotoxicity and downregulation of Bcl-2 expression in differentiated neuroblastoma SH-SY5Y cells.
MedLine Citation:
PMID:  19032555     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: It has been proposed that the volatile anesthetic isoflurane induces neuroprotection and that the endogenous opioid peptide dynorphin induces neurocytotoxicity in cells. The levels of dynorphin are often significantly elevated in neuropathophysiological conditions, and dynorphin can directly induce toxicity. However, the neuroprotective effects of isoflurane on dynorphin-induced cytotoxicity are still unclear. METHODS: In order to determine the effect of isoflurane on dynorphin-induced cytotoxicity in neuronal cells, we have designed a device wherein cultured human neuroblastoma SH-SY5Y cells can be exposed to isoflurane. Fully differentiated SH-SY5Y cells were obtained by treating the cells with retinoic acid for 6 days. We examined SH-SY5Y cell survival, apoptosis, and antiapoptotic protein expression by cell viability, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling stain, and Western blot analysis, respectively. RESULTS: After 16 h of dynorphin (10 microM) treatment, the SH-SY5Y cells showed significant cytotoxicity, apoptosis, and downregulation of the antiapoptotic Bcl-2 protein expression. These effects of dynorphin were significantly inhibited by isoflurane exposure for 32 h [pretreatment for 16 h and posttreatment (after dynorphin treatment) for 16 h]. CONCLUSION: Thus, our results suggest that isoflurane exerts neuroprotective effects in the case of dynorphin-induced pathophysiological disruption.
Authors:
G-J Wu; W-F Chen; C-S Sung; Y-H Jean; C-H Hung; F-A Chen; M-H Hsieh; Z-H Wen
Related Documents :
22912905 - Intravenous grafts of amniotic fluid-derived stem cells induce endogenous cell prolifer...
11266525 - Follicular lymphoma with marginal zone differentiation: microdissection demonstrates th...
17227835 - The bh3-mimetic gx15-070 synergizes with bortezomib in mantle cell lymphoma by enhancin...
12679905 - Tributyrin inhibits human gastric cancer sgc-7901 cell growth by inducing apoptosis and...
19840955 - Anti-apoptotic effects of z alpha1-antitrypsin in human bronchial epithelial cells.
2563675 - Ontogeny of somatostatin in cerebral cortex of macaque monkey: an immunohistochemical s...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-11
Journal Detail:
Title:  Acta anaesthesiologica Scandinavica     Volume:  53     ISSN:  1399-6576     ISO Abbreviation:  Acta Anaesthesiol Scand     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-08     Completed Date:  2009-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370270     Medline TA:  Acta Anaesthesiol Scand     Country:  England    
Other Details:
Languages:  eng     Pagination:  55-60     Citation Subset:  IM    
Affiliation:
Department of Anesthesiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects
Cell Differentiation*
Cell Line, Tumor
Down-Regulation / drug effects*
Dynorphins / toxicity*
Humans
Isoflurane / pharmacology*
Neuroblastoma / metabolism*,  pathology*
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 26675-46-7/Isoflurane; 74913-18-1/Dynorphins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Whole-blood aggregometry: are there any limits with regard to platelet counts?
Next Document:  Accuracy of Sequential Organ Failure Assessment (SOFA) scoring in clinical practice.