| Isoflurane alters energy substrate metabolism to preserve mechanical function in isolated rat hearts following prolonged no-flow hypothermic storage. | |
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MedLine Citation:
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PMID: 12552197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Isoflurane enhances mechanical function in hearts subject to normothermic global or regional ischemia. The authors examined the effectiveness of isoflurane in preserving mechanical function in hearts subjected to cardioplegic arrest and prolonged hypothermic no-flow storage. The role of isoflurane in altering myocardial glucose metabolism during storage and reperfusion during these conditions and the contribution of adenosine triphosphate-sensitive potassium (K(atp)) channel activation in mediating the functional and metabolic effects of isoflurane preconditioning was determined. METHODS: Isolated working rat hearts were subjected to cardioplegic arrest with St. Thomas' II solution, hypothermic no-flow storage for 8 h, and subsequent aerobic reperfusion. The consequences of isoflurane treatment were assessed during the following conditions: (1) isoflurane exposure before and during storage; (2) brief isoflurane exposure during early nonworking poststorage reperfusion; and (3) isoflurane preconditioning before storage. The selective mitochondrial and sarcolemmal K(atp) channel antagonists, 5-hydroxydecanoate and HMR 1098, respectively, were used to assess the role of K(atp) channel activation on glycogen consumption during storage in isoflurane-preconditioned hearts. RESULTS: Isoflurane enhanced recovery of mechanical function if present before and during storage. Isoflurane preconditioning was also protective. Isoflurane reduced glycogen consumption during storage under the aforementioned circumstances. Storage of isoflurane-preconditioned hearts in the presence of 5-hydroxydecanoate prevented the reduction in glycogen consumption during storage and abolished the beneficial effect of isoflurane preconditioning on recovery of mechanical function. CONCLUSIONS: Isoflurane provides additive protection of hearts subject to cardioplegic arrest and prolonged hypothermic no-flow storage and favorably alters energy substrate metabolism by modulating glycolysis during ischemia. The effects of isoflurane preconditioning on glycolysis during hypothermic no-flow storage appears to be associated with activation of mitochondrial K(atp) channels. |
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Authors:
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Barry A Finegan; Manoj Gandhi; Matthew R Cohen; Donald Legatt; Alexander S Clanachan |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anesthesiology Volume: 98 ISSN: 0003-3022 ISO Abbreviation: Anesthesiology Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-01-28 Completed Date: 2003-02-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1300217 Medline TA: Anesthesiology Country: United States |
Other Details:
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Languages: eng Pagination: 379-86 Citation Subset: AIM; IM |
Affiliation:
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Department of Anesthesiology, University of Alberta, Edmonton, Canada. bfinegan@ualberta.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Anesthetics, Inhalation / pharmacology* Animals Coronary Circulation / drug effects Energy Metabolism / drug effects Glucose / metabolism Glycogen / metabolism Heart / drug effects* Hypothermia, Induced Ischemic Preconditioning, Myocardial Isoflurane / pharmacology* Male Myocardial Contraction / drug effects Myocardium / metabolism* Rats Rats, Sprague-Dawley Specimen Handling |
| Chemical | |
Reg. No./Substance:
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0/Anesthetics, Inhalation; 26675-46-7/Isoflurane; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; 9005-79-2/Glycogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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