| Isoenzyme, restriction fragment length polymorphism, and random amplified polymorphic DNA characterization of Phaffia rhodozyma Miller et al. | |
| | |
MedLine Citation:
|
PMID: 7857798 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The validity of the species concept was examined with strains of Phaffia rhodozyma by comparing the isoenzyme profiles, ribosomal DNAs, and random amplified polymorphic DNA (RAPD) patterns of the strains. The isoenzyme profiles appeared to be more stable than the RAPD patterns or the electrophoretic karyotypes determined previously (A. Nagy, N. Garamszegi, C. Vágvölgyi, and L. Ferenczy, FEMS Microbiol. Lett., in press). The ribosomal DNA patterns revealed only a limited degree of variability, while RAPD analysis proved to be the most useful method for differentiating the strains studied. Strain CBS 5905T (T = type strain) produced characteristic RAPD patterns, which were different from those produced by the other strains. However, despite the high degree of variability observed, the isoenzyme data and the slightly variable ribosomal DNA hybridization profiles confirmed that the strains which we examined belong to one species. |
| | |
Authors:
|
J Varga; C Vágvölgyi; A Nagy; I Pfeiffer; L Ferenczy |
Related Documents
:
|
18944928 - Isolation, characterization, and distribution of a biocontrol fungus from cysts of hete... 17473888 - Adaptive changes of chemolithoautotrophic acidophilic sulfur-oxidizing bacteria during ... 11155928 - Polymorphism of the gene encoding a major polar tube protein ptp1 in two microsporidia ... 21088178 - Comparison of the fibers of fowl adenovirus a serotype 1 isolates from chickens with gi... 23315478 - Prevalence and mechanisms of β-lactam resistance in haemophilus haemolyticus. 16622058 - A cyanobacterial strain with all chromosomal rrna operons inactivated: a single nucleot... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: International journal of systematic bacteriology Volume: 45 ISSN: 0020-7713 ISO Abbreviation: Int. J. Syst. Bacteriol. Publication Date: 1995 Jan |
Date Detail:
|
Created Date: 1995-03-23 Completed Date: 1995-03-23 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0042143 Medline TA: Int J Syst Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 173-7 Citation Subset: IM |
Affiliation:
|
Department of Microbiology, Attila József University, Szeged, Hungary. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence DNA, Fungal / analysis* Gene Amplification* Isoenzymes / analysis* Molecular Sequence Data Polymorphism, Restriction Fragment Length* Yeasts / classification*, enzymology, genetics |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Fungal; 0/Isoenzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Microlunatus phosphovorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacter...
Next Document: Heterogeneity of genome sizes within the genus Spiroplasma.