Document Detail

Isoenzyme, restriction fragment length polymorphism, and random amplified polymorphic DNA characterization of Phaffia rhodozyma Miller et al.
MedLine Citation:
PMID:  7857798     Owner:  NLM     Status:  MEDLINE    
The validity of the species concept was examined with strains of Phaffia rhodozyma by comparing the isoenzyme profiles, ribosomal DNAs, and random amplified polymorphic DNA (RAPD) patterns of the strains. The isoenzyme profiles appeared to be more stable than the RAPD patterns or the electrophoretic karyotypes determined previously (A. Nagy, N. Garamszegi, C. Vágvölgyi, and L. Ferenczy, FEMS Microbiol. Lett., in press). The ribosomal DNA patterns revealed only a limited degree of variability, while RAPD analysis proved to be the most useful method for differentiating the strains studied. Strain CBS 5905T (T = type strain) produced characteristic RAPD patterns, which were different from those produced by the other strains. However, despite the high degree of variability observed, the isoenzyme data and the slightly variable ribosomal DNA hybridization profiles confirmed that the strains which we examined belong to one species.
J Varga; C Vágvölgyi; A Nagy; I Pfeiffer; L Ferenczy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of systematic bacteriology     Volume:  45     ISSN:  0020-7713     ISO Abbreviation:  Int. J. Syst. Bacteriol.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-03-23     Completed Date:  1995-03-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0042143     Medline TA:  Int J Syst Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  173-7     Citation Subset:  IM    
Department of Microbiology, Attila József University, Szeged, Hungary.
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MeSH Terms
Base Sequence
DNA, Fungal / analysis*
Gene Amplification*
Isoenzymes / analysis*
Molecular Sequence Data
Polymorphism, Restriction Fragment Length*
Yeasts / classification*,  enzymology,  genetics
Reg. No./Substance:
0/DNA, Fungal; 0/Isoenzymes

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