Document Detail


Isoelectric focusing in low-denaturing media: visualization in renal disease of variation of the isoelectric point of albumin not related to a remarkable conformational variation.
MedLine Citation:
PMID:  2420813     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The isoelectric properties of serum and urinary albumin from normal subjects and patients with nephrotic syndrome have been investigated in various conditions of denaturation, obtained by using urea (0-8 M) as a support in isoelectric focusing. In normal human serum, albumin is rather acidic (pI = 4.7) when focused in glycerol while the denatured form obtained by exposing the protein to 8 M urea has a much higher pI (6.1). Albumin from nephrotic patients is acidic in glycerol but at very low levels of urea (2M) it shifts from pI 4.7 to pI 6.1; the same effect has been induced by treating albumin with activated charcoal at low pH. In order to obtain more information on urea-induced changes, we have recorded the circular dichroic spectra of albumin when exposed to the concentration of urea used in gels, and we found that no conformational transition occurs for urea concentrations less than 5 M. Taken together, these observations reveal that variation of the pI of albumin in nephrotic syndrome occurs mainly due to a dissociating effect of urea on charged substances bound to this protein.
Authors:
G Candiano; G M Ghiggeri; G Delfino; C Queirolo; G Vecchio
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  374     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-04-28     Completed Date:  1986-04-28     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  279-88     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Albumins / analysis*
Albuminuria / metabolism
Circular Dichroism
Humans
Isoelectric Focusing
Kidney Diseases / metabolism*
Nephrotic Syndrome / blood
Protein Conformation
Serum Albumin / analysis
Staining and Labeling
Chemical
Reg. No./Substance:
0/Albumins; 0/Serum Albumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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