| Isoelectric focusing in low-denaturing media: visualization in renal disease of variation of the isoelectric point of albumin not related to a remarkable conformational variation. | |
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MedLine Citation:
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PMID: 2420813 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The isoelectric properties of serum and urinary albumin from normal subjects and patients with nephrotic syndrome have been investigated in various conditions of denaturation, obtained by using urea (0-8 M) as a support in isoelectric focusing. In normal human serum, albumin is rather acidic (pI = 4.7) when focused in glycerol while the denatured form obtained by exposing the protein to 8 M urea has a much higher pI (6.1). Albumin from nephrotic patients is acidic in glycerol but at very low levels of urea (2M) it shifts from pI 4.7 to pI 6.1; the same effect has been induced by treating albumin with activated charcoal at low pH. In order to obtain more information on urea-induced changes, we have recorded the circular dichroic spectra of albumin when exposed to the concentration of urea used in gels, and we found that no conformational transition occurs for urea concentrations less than 5 M. Taken together, these observations reveal that variation of the pI of albumin in nephrotic syndrome occurs mainly due to a dissociating effect of urea on charged substances bound to this protein. |
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Authors:
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G Candiano; G M Ghiggeri; G Delfino; C Queirolo; G Vecchio |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 374 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-04-28 Completed Date: 1986-04-28 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 279-88 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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analysis* Albuminuria / metabolism Circular Dichroism Humans Isoelectric Focusing Kidney Diseases / metabolism* Nephrotic Syndrome / blood Protein Conformation Serum Albumin / analysis Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Serum Albumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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