Document Detail


Isocitric and citric acid in human prostatic and seminal fluid: implications for prostatic metabolism and secretion.
MedLine Citation:
PMID:  8115279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human prostatic secretion is remarkably rich in citric acid but the mechanisms to account for this accumulation are not well understood. One factor may be the extent of citrate oxidation to isocitrate, catalyzed by aconitase. The citrate-to-isocitrate ratio will help characterize the relative significance of this reaction in prostatic production and secretion of citrate. Isocitric acid and citric acid were measured in samples of seminal fluid and expressed prostatic secretion (EPS). A constant ratio between citrate and isocitrate of about 33:1 was found (r = 0.93, P < 0.0001) despite the wide variation in concentrations. Citrate ranged from 1 to 180 mM in EPS and from 13 to 50 mM in seminal fluid while isocitrate varied between 0 to 4.8 mM in EPS and from 0.4 to 1.5 mM in seminal fluid. Isocitrate is present in EPS and semen at much higher levels than found in most other animal or plant tissues or fluids and may be actively secreted by the same mechanism as citrate. The high citrate to isocitrate ratio of about 33:1, compared to the expected value of about 10:1, supports suggestions that citrate to isocitrate oxidation by aconitase is a rate limiting step in prostatic citrate metabolism. A low aconitase activity will therefore play a significant role in enabling accumulation of high citrate levels in prostatic epithelia and acini.
Authors:
J P Kavanagh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Prostate     Volume:  24     ISSN:  0270-4137     ISO Abbreviation:  Prostate     Publication Date:  1994  
Date Detail:
Created Date:  1994-03-29     Completed Date:  1994-03-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8101368     Medline TA:  Prostate     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  139-42     Citation Subset:  IM    
Affiliation:
Department of Urology, University Hospital of South Manchester, England.
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MeSH Terms
Descriptor/Qualifier:
Citrates / analysis*,  metabolism
Citric Acid
Humans
Isocitrate Dehydrogenase / pharmacology
Isocitrates / analysis*,  metabolism
Male
NADP / metabolism
Oxidation-Reduction
Prostate / chemistry,  secretion*
Semen / chemistry*
Trichloroacetic Acid / pharmacology
Chemical
Reg. No./Substance:
0/Citrates; 0/Isocitrates; 320-77-4/isocitric acid; 53-59-8/NADP; 76-03-9/Trichloroacetic Acid; 77-92-9/Citric Acid; EC 1.1.1.41/Isocitrate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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