Document Detail


Isocaloric modification of dietary lipids influences intestinal brush border membrane composition in diabetic rats.
MedLine Citation:
PMID:  1802479     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Variations in the dietary fatty acid composition and cholesterol content are associated with alterations in the intestinal uptake of hexoses and lipids in control and diabetic rats. Changes in the composition of the brush membrane (BBM) lipids may provide a possible mechanism for the observed alterations in transport properties. Accordingly, control and streptozotocin diabetic animals were fed one of four isocaloric semisynthetic diets for two weeks: beef tallow with low cholesterol, beef tallow with high cholesterol, fish oil with low cholesterol or fish oil with high cholesterol. BBM were prepared and assessed for marker enzyme activity and lipid composition. Fish oil feeding was associated with a reduction in total phospholipid content in control and diabetic jejunal and ileal BBM; this fall in total phospholipids was due to a reduction in BBM sphingomyelin. Cholesterol supplementation increased control jejunal BBM sucrase activity in animals fed beef tallow but reduced sucrase activity in animals fed fish oil. In fish oil fed diabetic animals, jejunal and ileal BBM alkaline phosphatase activity was increased with cholesterol supplementation. The elevation in BBM total phospholipids (phosphatidylethanolamine) associated with diabetes in beef tallow fed animals was not observed in the jejunal BBM of animals fed fish oil or in the ileal BBM of animals fed fish oil with high cholesterol. Thus, (a) feeding an omega-3 polyunsaturated fatty acid diet (fish oil) reduced total phospholipid content in BBM of control and diabetic animals, primarily due to a reduction in sphingomyelin; and (b) feeding an omega-3 polyunsaturated fatty acid diet or dietary cholesterol supplementation alter the activity of BBM enzymes. These results suggest that variations in dietary fat composition and the associated changes in BBM composition and enzyme activity contribute to altered intestinal function in diabetes.
Authors:
M Keelan; A B Thomson; A A Wierzbicki; E Wierzbicki; R Rajotte; M T Clandinin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetes research (Edinburgh, Scotland)     Volume:  16     ISSN:  0265-5985     ISO Abbreviation:  Diabetes Res.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1992-04-28     Completed Date:  1992-04-28     Revised Date:  2007-09-11    
Medline Journal Info:
Nlm Unique ID:  8502339     Medline TA:  Diabetes Res     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  127-38     Citation Subset:  IM    
Affiliation:
Nutrition and Metabolism Research Group, University of Alberta, Edmonton, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cholesterol / metabolism
Cholesterol, Dietary / pharmacology*
Diabetes Mellitus, Experimental / metabolism*,  pathology
Dietary Fats / pharmacology*
Energy Intake
Fats / pharmacology
Female
Fish Oils / pharmacology
Intestinal Mucosa / drug effects,  metabolism*,  ultrastructure
Membrane Lipids / metabolism*
Microvilli / drug effects,  metabolism*,  ultrastructure
Phospholipids / metabolism
Rats
Rats, Inbred Strains
Reference Values
Sucrase / metabolism
Chemical
Reg. No./Substance:
0/Cholesterol, Dietary; 0/Dietary Fats; 0/Fats; 0/Fish Oils; 0/Membrane Lipids; 0/Phospholipids; 57-88-5/Cholesterol; 61789-97-7/tallow; EC 3.2.1.48/Sucrase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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