Document Detail


Islet1 derivatives in the heart are of both neural crest and second heart field origin.
MedLine Citation:
PMID:  22394517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE: Islet1 (Isl1) has been proposed as a marker of cardiac progenitor cells derived from the second heart field and is utilized to identify and purify cardiac progenitors from murine and human specimens for ex vivo expansion. The use of Isl1 as a specific second heart field marker is dependent on its exclusion from other cardiac lineages such as neural crest.
OBJECTIVE: Determine whether Isl1 is expressed by cardiac neural crest.
METHODS AND RESULTS: We used an intersectional fate-mapping system using the RC::FrePe allele, which reports dual Flpe and Cre recombination. Combining Isl1(Cre/+), a SHF driver, and Wnt1::Flpe, a neural crest driver, with Rc::FrePe reveals that some Isl1 derivatives in the cardiac outflow tract derive from Wnt1-expressing neural crest progenitors. In contrast, no overlap was observed between Wnt1-derived neural crest and an alternative second heart field driver, Mef2c-AHF-Cre.
CONCLUSIONS: Isl1 is not restricted to second heart field progenitors in the developing heart but also labels cardiac neural crest. The intersection of Isl1 and Wnt1 lineages within the heart provides a caveat to using Isl1 as an exclusive second heart field cardiac progenitor marker and suggests that some Isl1-expressing progenitor cells derived from embryos, embryonic stem cultures, or induced pluripotent stem cultures may be of neural crest lineage.
Authors:
Kurt A Engleka; Lauren J Manderfield; Rachael D Brust; Li Li; Ashley Cohen; Susan M Dymecki; Jonathan A Epstein
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-06
Journal Detail:
Title:  Circulation research     Volume:  110     ISSN:  1524-4571     ISO Abbreviation:  Circ. Res.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-30     Completed Date:  2012-07-05     Revised Date:  2013-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  922-6     Citation Subset:  IM    
Affiliation:
Department of Cell and Developmental Biology, Cardiovascular Institute and Institute for Regenerative Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers
Cell Lineage*
Green Fluorescent Proteins / genetics
Heart / embryology*
LIM-Homeodomain Proteins / genetics,  metabolism*
Mice
Mice, Transgenic
Models, Animal
Myocardium / cytology,  metabolism*
Neural Crest / cytology,  embryology*
Stem Cells / cytology,  metabolism
Transcription Factors / genetics,  metabolism*
Wnt1 Protein / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
HL062974/HL/NHLBI NIH HHS; HL095634/HL/NHLBI NIH HHS; R01 HD051936/HD/NICHD NIH HHS; R01 HL062974/HL/NHLBI NIH HHS; R01 HL095634/HL/NHLBI NIH HHS; R01HD051936/HD/NICHD NIH HHS; R21 DA023643/DA/NIDA NIH HHS; R21 MH083613/MH/NIMH NIH HHS; R21DA023643-01/DA/NIDA NIH HHS; R21MH083613/MH/NIMH NIH HHS; T32 GM007226/GM/NIGMS NIH HHS; U01 HL100405/HL/NHLBI NIH HHS; U01 HL100405/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/LIM-Homeodomain Proteins; 0/Transcription Factors; 0/Wnt1 Protein; 0/insulin gene enhancer binding protein Isl-1; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections
Erratum In:
Circ Res. 2012 May 25;110(11):e90

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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