| Islet loss and alpha cell expansion in type 1 diabetes induced by multiple low-dose streptozotocin administration in mice. | |
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MedLine Citation:
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PMID: 10750039 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the alpha cell population during the development of type 1 diabetes following multiple low-dose streptozotocin administration in mice. For this purpose C57BL/Ks male mice were injected with streptozotocin (40 mg/kg body weight for 5 days). Development of hyperglycemia was monitored over 28 days and a morphometric analysis of islet endocrine cells was performed. A reduction of islet cell area was observed after two injections of streptozotocin. The subsequent decrease of the area throughout the study period averaged 35%. Insulin-positive beta cells gradually disappeared from the identified islets. Hyperglycemia was present from day 7 onwards and in parallel with hyperglycemia, insulitis developed. An analysis of the alpha cell number per islet area revealed a 2- to 3-fold increase in this cell population, with the highest value on day 21. Confocal microscopy analysis of the ICA 512 protein tyrosine phosphatase revealed strong expression in the alpha cells at day 21, suggesting high secretory activity in the diabetic state. It is concluded that multiple low-dose streptozotocin treatment of C57BL/Ks male mice causes the disappearance of a fraction of the islets of Langerhans. In the remaining islet tissue an expansion of alpha cells occurs, reflecting a loss of intraislet beta cells as well as a regeneration of alpha cells. |
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Authors:
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Z Li; F A Karlsson; S Sandler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of endocrinology Volume: 165 ISSN: 0022-0795 ISO Abbreviation: J. Endocrinol. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-05-11 Completed Date: 2000-05-11 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 93-9 Citation Subset: IM |
Affiliation:
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Department of Medical Sciences, Uppsala University, Uppsala, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Bacterial Agents Autoantigens Biological Markers / analysis Cell Count / drug effects Diabetes Mellitus, Experimental / chemically induced, metabolism* Islets of Langerhans / cytology, drug effects* Male Membrane Proteins / analysis Mice Mice, Inbred C57BL Microscopy, Confocal Protein Tyrosine Phosphatase, Non-Receptor Type 1 Protein Tyrosine Phosphatases / analysis Receptor-Like Protein Tyrosine Phosphatases, Class 8 Streptozocin |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Autoantigens; 0/Biological Markers; 0/Membrane Proteins; 18883-66-4/Streptozocin; EC 3.1.3.48/Protein Tyrosine Phosphatase, Non-Receptor Type 1; EC 3.1.3.48/Protein Tyrosine Phosphatases; EC 3.1.3.48/Ptprn protein, mouse; EC 3.1.3.48/Receptor-Like Protein Tyrosine Phosphatases, Class 8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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