Document Detail


Islet hyperperfusion during prediabetic phase in OLETF rats, a model of type 2 diabetes.
MedLine Citation:
PMID:  12145167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although it has been hypothesized that initial hyperperfusion followed by late hypoperfusion in islet circulation occurs in rodent models of type 2 diabetes, islet blood flow has not been measured during prediabetic phase. We studied islet blood flow in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model of slowly progressive obese type 2 diabetes. Islet blood flow was measured by the two-color microsphere method under anesthesia at different ages. Islet blood flow was significantly higher in young OLETF rats compared with control Long-Evans Tokushima Otsuka (LETO) rats when the former were not obese or diabetic and had normal insulin secretion at 5 weeks of age (LETO 4.6 +/- 1.1, OLETF 8.8 +/- 1.2 ml. min(-1). mg(- 1), P < 0.01). At 6 months of age, islet hyperperfusion was observed in OLETF rats, and >40% of whole pancreatic blood flow was diverted into islets in OLETF rats. Prevention of obesity by food restriction increased basal islet blood flow. On the other hand, long-term hyperglycemia induced by sucrose feeding decreased fractional islet blood flow as well as glucose-stimulated islet blood flow. Our results indicate that hyperperfusion is present during the preobese and prediabetic phase in our type 2 diabetes rats.
Authors:
Masanori Iwase; Yuji Uchizono; Kenji Tashiro; Daisuke Goto; Mitsuo Iida
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetes     Volume:  51     ISSN:  0012-1797     ISO Abbreviation:  Diabetes     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-29     Completed Date:  2002-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2530-5     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. iwase@intmed2.med.kyushu-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Diabetes Mellitus, Type 2 / physiopathology*
Diet, Reducing
Dietary Sucrose / pharmacology
Disease Models, Animal
Insulin / blood*
Islets of Langerhans / blood supply,  drug effects,  physiopathology*
Male
Pancreas / blood supply
Perfusion
Prediabetic State / physiopathology*
Rats
Rats, Inbred OLETF
Rats, Long-Evans
Regional Blood Flow / drug effects
Species Specificity
omega-N-Methylarginine / pharmacology
Chemical
Reg. No./Substance:
0/Dietary Sucrose; 11061-68-0/Insulin; 17035-90-4/omega-N-Methylarginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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