Document Detail


Islet cell surface antibodies by an ELISA method in diabetic and nondiabetic patients.
MedLine Citation:
PMID:  1865079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Islet cell surface antibodies (ICSA) were investigated by an ELISA method using a commercial kit in 146 subjects with and without islet cell antibodies (ICA): 28 with insulin-dependent diabetes mellitus (IDDM), 24 with noninsulin-dependent diabetes mellitus (NIDDM), 22 first-degree relatives (FDR) of IDDM patients, 31 organ-specific autoimmune patients (OSAP), 21 nonautoimmune hospitalized patients (NAP), and 20 ICA-negative normal controls. Furthermore, insulin autoantibodies (IAA) were evaluated in 87 of these subjects. ICSA were found in 11% of IDDM patients and in 14% of their FDR, in 4% of NIDDM patients, in 10% of OSAP, in 10% of NAP, and in 5% of normal controls. After absorption with rat liver powder, ICSA were detected in 7% of IDDM patients, in 5% of their FDR, in 4% of NIDDM, in 6% of OSAP, in 5% of NAP and in none of normal controls. ICSA were also detected in 4% of IAA-positive compared to 3% of IAA-negative sera. Neither correlation was found between ICSA and ICA in each group of subjects, nor between ICSA and IAA, suggesting that these autoantibodies recognize different pancreatic targets. Moreover, no significant difference was observed for ICSA prevalence in the various groups of patients studied when compared with normal controls. The prevalence of ICSA assessed by this ELISA method has been compared to that reported by other workers, who employed different techniques.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
C Betterle; F Presotto; L Magrin; A Caretto; B Pedini; R Zanchetta
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of endocrinological investigation     Volume:  14     ISSN:  0391-4097     ISO Abbreviation:  J. Endocrinol. Invest.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-09-06     Completed Date:  1991-09-06     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7806594     Medline TA:  J Endocrinol Invest     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  293-7     Citation Subset:  IM    
Affiliation:
Istituto di Semeiotica Medica, Università di Padova, Italy.
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MeSH Terms
Descriptor/Qualifier:
Addison Disease / immunology
Autoantibodies / blood*
Autoimmune Diseases / immunology*
Biological Markers / blood
Chi-Square Distribution
Diabetes Mellitus, Type 1 / genetics,  immunology*
Diabetes Mellitus, Type 2 / immunology*
Enzyme-Linked Immunosorbent Assay
Graves Disease / immunology
Humans
Immunoglobulin G / analysis
Islets of Langerhans / immunology*
Thyroiditis, Autoimmune / immunology
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Biological Markers; 0/Immunoglobulin G

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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