Document Detail


Islet cell antigens. Extraction studies and ELISA analysis.
MedLine Citation:
PMID:  3292332     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Whole human and bovine pancreases were extracted in 20 mM Tris-HCl buffer without detergents and fractionated by high-speed centrifugation. The 80,000 x g supernatant was used to coat microtiter plates at a concentration of 5 micrograms protein/ml in phosphate-buffered saline. This solid-phase ELISA system was used for the detection of islet cell antigens defined by a series of monoclonal islet cell antibodies (HISL-1, -4, -5, -8, -14, and -19 and 4F2, 3G5, and A2B5). Both glycoprotein and glycolipid islet cell antigens in the total pancreatic extracts were detected by the monoclonal islet cell antibody in the ELISA system, indicating that epitope preservation had occurred during the extraction procedure. There was a good correlation between islet cell antigen quantitated by the ELISA system and the corresponding islet immunohistochemical reaction. Studies along these lines have the potential to facilitate the design of large-scale protocols for the purification of diabetes-related islet cell antigens to homogeneity.
Authors:
R Moncayo; K Kirsch; T Pasquarello; E F Pfeiffer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Diabetes     Volume:  37     ISSN:  0012-1797     ISO Abbreviation:  Diabetes     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-08-24     Completed Date:  1988-08-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1137-43     Citation Subset:  AIM; IM    
Affiliation:
Department of Internal Medicine, University of Ulm, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use
Antigens, Differentiation / isolation & purification*
Cattle
Enzyme-Linked Immunosorbent Assay / methods*
Humans
Islets of Langerhans / analysis,  immunology*
Methods
Grant Support
ID/Acronym/Agency:
AM-01378/AM/NIADDK NIH HHS; AM-37148/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Differentiation

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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