Document Detail


Ischaemic preconditioning-regulated miR-21 protects heart against ischaemia/reperfusion injury via anti-apoptosis through its target PDCD4.
MedLine Citation:
PMID:  20219857     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: The aims of the present study are to determine the miRNA expression signature in rat hearts after ischaemic preconditioning (IP) and to identify an IP-regulated miRNA, miR-21, in IP-mediated cardiac protection, and the potential cellular and molecular mechanisms involved.
METHODS AND RESULTS: The miRNA expression signature was investigated in rat hearts. Among the 341 arrayed miRNAs, 40 miRNAs were differentially expressed (21 up and 19 down) in rat hearts with IP, compared with their controls. Some of these differentially expressed miRNAs were further verified by quantitative reverse transcriptase-polymerase chain reaction. Remarkably, miR-21 was one of most upregulated miRNAs in hearts after IP. In vivo, IP-mediated cardiac protection against ischaemia/reperfusion injury was inhibited by knockdown of cardiac miR-21. In cultured cardiac myocytes, we identified that miR-21 also had a protective effect on hypoxia/reoxygenation-induced cell apoptosis that was associated with its target gene, programmed cell death 4. The protective effect of miR-21 on cardiac cell apoptosis was further confirmed in rat hearts after ischaemia/reperfusion injury in vivo.
CONCLUSION: The results suggest that miRNAs are involved in IP-mediated cardiac protection. Identifying the roles of IP-regulated miRNAs in cardiac protection may provide novel therapeutic and preventive targets for ischaemic heart disease.
Authors:
Yunhui Cheng; Ping Zhu; Jian Yang; Xiaojun Liu; Shimin Dong; Xiaobin Wang; Bao Chun; Jian Zhuang; Chunxiang Zhang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-03-10
Journal Detail:
Title:  Cardiovascular research     Volume:  87     ISSN:  1755-3245     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-16     Completed Date:  2010-11-03     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  431-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Apoptosis / genetics*
Apoptosis Regulatory Proteins / genetics*,  metabolism
Cell Hypoxia
Cells, Cultured
Cytoprotection / genetics
Disease Models, Animal
Gene Expression Profiling / methods
Ischemic Preconditioning, Myocardial*
Male
MicroRNAs / metabolism*
Myocardial Infarction / genetics,  pathology,  prevention & control*
Myocardial Reperfusion Injury / genetics,  pathology,  prevention & control*
Myocardium / metabolism*,  pathology
Oligonucleotide Array Sequence Analysis
Oligonucleotides, Antisense / metabolism
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Transfection
Grant Support
ID/Acronym/Agency:
HL080133/HL/NHLBI NIH HHS; HL095707/HL/NHLBI NIH HHS; R01 HL080133/HL/NHLBI NIH HHS; R01 HL080133-05/HL/NHLBI NIH HHS; R01 HL095707/HL/NHLBI NIH HHS; R01 HL095707-03/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/MicroRNAs; 0/Oligonucleotides, Antisense; 0/Pdcd4 protein, rat; 0/mirn21 microRNA, rat
Comments/Corrections
Comment In:
Cardiovasc Res. 2010 Aug 1;87(3):397-400   [PMID:  20562424 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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