Document Detail


Ischemic preconditioning and intracellular pH: a 31P NMR study in the isolated rat heart.
MedLine Citation:
PMID:  9038977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of these studies was to investigate whether manipulation of intracellular pH affects preconditioning in isolated buffer-perfused rat hearts. Control and preconditioned [PC; 3 min of ischemia (I) + 3 min of reperfusion (R) + 5 min of I + 5 min of R or 4 x (5 min of I + 5 min of R)] hearts were subjected to two different protocols expected to alter intracellular pH during the sustained ischemic insult: 1) increased extracellular buffering capacity with the addition of 25 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) to the buffer to alleviate acidosis and 2) increased preischemic glycogen content to exacerbate acidosis. All hearts were subjected to 40 min of I + 40 min of R. 31P nuclear magnetic resonance was used to measure ATP, phosphocreatine, Pi, and intracellular pH. Despite a significantly better recovery of function in all PC groups, there were no significant differences in intracellular pH (rate-pressure product = 60 +/- 5, 66 +/- 10, 42 +/- 5, and 57 +/- 8% of baseline in PC, 4 x 5 PC, PC + HEPES, and PC fasted hearts, respectively, compared with 36 +/- 9, 17 +/- 7, and 20 +/- 10% of baseline in control, control + HEPES, and control fasted hearts, respectively; pH at end ischemia: control, 6.31 +/- 0.02; PC, 6.35 +/- 0.03; 4 x 5 PC, 6.35 +/- 0.04; control + HEPES, 6.40 +/- 0.10; PC + HEPES, 6.56 +/- 0.07: control fasted, 6.46 +/- 0.03; PC fasted, 6.43 +/- 0.01). No significant differences were observed among groups in ATP, phosphocreatine, or Pi on reperfusion. Thus the mechanism of preconditioning in glucose-perfused hearts does not depend on an alleviation of intracellular acidosis during the sustained ischemic period. Furthermore, under the conditions of this study, intracellular pH during ischemia did not predict functional recovery on reperfusion.
Authors:
A C Cave; P B Garlick
Related Documents :
10067977 - Riluzole improves functional recovery after ischemia in the rat retina.
22421027 - Two-dimensional strain rate and doppler tissue myocardial velocities: analysis by echoc...
8902527 - A hydroxylated analog of the beta-adrenoceptor antagonist, carvedilol, affords exceptio...
22915967 - Angiotensin ii type 1 receptor antagonists in the treatment of hypertension in elderly ...
6775747 - Chronic stable asthma and the normal arterial pressure of carbon dioxide in hypoxia.
3802437 - The association of postural changes in systolic blood pressure and mortality in persons...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  272     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-31     Completed Date:  1997-03-31     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H544-52     Citation Subset:  IM    
Affiliation:
Division of Radiological Sciences, Guy's Hospital, London, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Energy Metabolism
Glycogen / metabolism
Heart / physiology
Hydrogen / metabolism*
Hydrogen-Ion Concentration
Intracellular Membranes / metabolism*
Ischemic Preconditioning, Myocardial*
Magnetic Resonance Spectroscopy
Male
Myocardial Contraction
Myocardium / metabolism*
Phosphates / metabolism
Phosphorus
Rats
Rats, Wistar
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Phosphates; 1333-74-0/Hydrogen; 7723-14-0/Phosphorus; 9005-79-2/Glycogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Membrane lipid order of human red blood cells is altered by physiological levels of hydrostatic pres...
Next Document:  Capacitive function of the heart: influence of acute changes in heart volume on mean right atrial pr...