| Ischemic postconditioning does not attenuate ischemia-reperfusion injury of rabbit small intestine. | |
| | |
MedLine Citation:
|
PMID: 20210969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To determine whether ischemic postconditioning can attenuate intestinal ischemia-reperfusion (I-R) injury and has a beneficial effect on tissue blood flow during reperfusion. STUDY DESIGN: In vivo experimental study. ANIMALS: New Zealand White rabbits (n=6). METHODS: Rabbits were anesthetized with pentobarbital, to avoid the preconditioning effects of volatile anesthetics, and ventilated with room air. Rectal temperature, hemodynamics, and normocapnia were maintained. After celiotomy, 3 jejunal segments were isolated in each rabbit for the following groups: (1) control, (2) I-R, and (3) I-R with postconditioning. I-R was induced by a 45-minute occlusion of the segment jejunal artery followed by 2-hour reperfusion. The postconditioning segment had 4 cycles of 30-second reperfusion and 30-second reocclusion during the initial 4 minutes of reperfusion. Stable isotope-labeled microspheres were used to measure intestinal blood flow at baseline, end occlusion, and end reperfusion. At the end of reperfusion, intestine segments were harvested and the rabbits euthanatized. A semiquantitative histopathologic evaluation (0-5) was conducted by a single, blinded observer. Wet-to-dry weight ratios were calculated to assess intestinal edema. RESULTS: There was no significant difference in grade of necrosis, tissue wet-to-dry weight ratios, or blood flow at any time point between ischemic and postconditioning groups. CONCLUSIONS: Ischemic postconditioning was ineffective in this model of intestinal I-R. CLINICAL RELEVANCE: Further experimental studies will need to be performed before clinical application of postconditioning for intestinal ischemia. |
| | |
Authors:
|
Brian Bretz; Cheryl Blaze; Nicola Parry; Raymond K Kudej |
Related Documents
:
|
14512289 - Study of intestinal flow by combined videofluoroscopy, manometry, and multiple intralum... 17306459 - Fatal cytauxzoonosis in captive-reared lions in brazil. 7048829 - Intestinal hemodynamic effects of varying the route of infusion of live e. coli bacteri... 734239 - Effect of furosemide on mesenteric blood flow in the dog. 747469 - Motility and hemodynamics of the canine gastrointestinal tract. stimulation by pentagas... 8619699 - The gastrointestinal tract: an underestimated organ as demonstrated in an experimental ... 14512289 - Study of intestinal flow by combined videofluoroscopy, manometry, and multiple intralum... 443769 - Diffuse cerebral ischemia in the cat: iii. neuropathological sequelae of severe ischemia. 19233149 - Evaluation of the xe-5000 for the automated analysis of blood cells in cerebrospinal fl... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Veterinary surgery : VS Volume: 39 ISSN: 1532-950X ISO Abbreviation: Vet Surg Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-03-09 Completed Date: 2010-05-07 Revised Date: 2011-04-25 |
Medline Journal Info:
|
Nlm Unique ID: 8113214 Medline TA: Vet Surg Country: United States |
Other Details:
|
Languages: eng Pagination: 216-23 Citation Subset: IM |
Affiliation:
|
Department of Clinical Sciences, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Hemodynamics Intestine, Small* / blood supply, pathology Ischemic Preconditioning / veterinary* Jejunal Diseases / pathology, prevention & control, veterinary Rabbits Regional Blood Flow Reperfusion Injury / pathology, prevention & control, veterinary* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro biomechanical evaluation and comparison of FiberWire, FiberTape, OrthoFiber, and nylon lead...
Next Document: Mandibular rim excision in seven dogs.