| Ischemic postconditioning inhibits apoptosis after acute myocardial infarction in pigs. | |
| | |
MedLine Citation:
|
PMID: 20961830 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: Recent studies have shown that ischemic postconditioning reduces myocardial ischemia-reperfusion (I/R) injury; however, the effects of inhibiting apoptosis on cardioprotection induced by ischemic postconditioning remain to be determined. The objective of this study was to investigate whether ischemic postconditioning attenuates myocardial I/R injury by reduced apoptosis in a closed-chest pig model of acute myocardial infarction. METHODS: Diannan small-ear pigs were randomly divided into 3 groups (5/group): (1) The sham group underwent a sham operation without ischemia; (2) the I/R group received 60 minutes of ischemia and 72 hours of reperfusion; and (3) the ischemic postconditioning (Postcond) group was treated the same as the I/R group except that the pigs received 8 cycles of 30 seconds of reperfusion and 30 seconds of ischemia at the onset of reperfusion. After 72 hours of reperfusion, infarct size was measured by 2,3,5-triphenyltetrazolium chloride staining. Apoptotic cells in the peri-infarct myocardium were evaluated with the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method, and apoptosis-related molecules were studied with western blotting analysis. RESULTS: After 72 hours of reperfusion, mean (±SEM) infarct size was significantly smaller in the Postcond group than in the I/R group (23.26% ± 3.13% versus 10.89% ± 2.02%, P < .05). Apoptotic myocytes in the peri-infarct region were lower in the Postcond group than in the I/R group (15.31% ± 4.58% versus 33.83% ± 4.44%, P < .05). This decrease in the extent of apoptosis was accompanied by a significant decrease in Bax expression (0.306 ± 0.075 versus 0.433 ± 0.102 for the I/R group; P < .05) and a significant increase in Bcl-2 expression (1.801 ± 0.227 versus 1.267 ± 0.308 for the I/R group; P < .05). CONCLUSIONS: In a clinically relevant closed-chest pig model of myocardial infarction, these data suggest the following: (1) Ischemic postconditioning reduces infarct size following prolonged reperfusion, and (2) this cardioprotective effect is likely achieved via antiapoptotic mechanisms. |
| | |
Authors:
|
Haimei Sun; Tao Guo; Liu Liu; Zhuo Yu; Wangbing Xu; Wenhui Chen; Lijuan Shen; Jiaping Wang; Xingkui Dou |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: The heart surgery forum Volume: 13 ISSN: 1522-6662 ISO Abbreviation: Heart Surg Forum Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-10-21 Completed Date: 2011-04-07 Revised Date: 2011-08-05 |
Medline Journal Info:
|
Nlm Unique ID: 100891112 Medline TA: Heart Surg Forum Country: United States |
Other Details:
|
Languages: eng Pagination: E305-10 Citation Subset: IM |
Affiliation:
|
Department of ICU, The First Affiliated Hospital of Kunming Medical College, 295 Xin-Chang Road, Kunming, P. R. China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis* Blotting, Western Disease Models, Animal Electrocardiography Follow-Up Studies Heart Ventricles / metabolism, pathology* In Situ Nick-End Labeling Ischemic Preconditioning, Myocardial / methods* Myocardial Infarction / metabolism, pathology, therapy* Myocardium / metabolism, pathology* Proto-Oncogene Proteins c-bcl-2 / biosynthesis Swine bcl-2-Associated X Protein / biosynthesis |
| Chemical | |
Reg. No./Substance:
|
0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Modification of a hybrid technique for closure of muscular ventricular septal defects in a pig model...
Next Document: Pericardial reconstruction using an extracellular matrix implant correlates with reduced risk of pos...