Document Detail


Ischemic postconditioning does not provide cardioprotection from long-term ischemic injury in isolated male or female rat hearts.
MedLine Citation:
PMID:  20934717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Ischemic postconditioning (PoC) is a cardio-protective strategy in which initial reperfusion is interrupted by episodes of ischemia. It is unclear whether PoC can be achieved in the Langendorff perfused rat heart model. We investigated (1) whether postconditioning occurs in Langendorff perfused rat heart and (2) whether there is a gender-specific response to PoC.
MATERIALS AND METHODS: Male/female rat hearts (n = 8/group) were subjected to 30 min of equilibration, 30 min of ischemia, and 120 min of reperfusion (Control). PoC was induced by 6 cycles (PoC 6c10s), 3 cycles (PoC 3c10s), or 2 cycles (PoC 2c10s) of 10 s reperfusion/10 s ischemia. Rate pressure product (RPP) and infarct size were measured. Male rats (n = 7/group) were subjected in vivo to 30 min left coronary ligation followed by 24 h of reperfusion (Control) or PoC 6c10s and 24 h of reperfusion.
RESULTS: Recovery of RPP was 18% ± 4% in male Control versus 17% ± 2% for 6c10s, 16% ± 1% for 3c10s, and 15% ± 3% for 2c10s. Female Control hearts recovered 25% ± 3% of their RPP versus 21% ± 2% for 6c10s. Infarct size was 25% ± 3% for male Control versus 26% ± 3% for 6c10s, 30% ± 2% for 3c10s, 28% ± 1% for 2c10s, and 30% ± 2% for female Control versus 29% ± 2% in 6c10s. In vivo infarct size for Control and PoC 6c10s was 44% ± 3% and 28% ± 5%, respectively (P < 0.05).
CONCLUSIONS: In the Langendorff perfused rat hearts, none of the PoC protocols improved myocardial tolerance to ischemia reperfusion injury nor decreased infarct size; however, in vivo postconditioning did confer protection. The lack of protection in the isolated hearts was not gender specific.
Authors:
Daniel S Lee; Gregory E Steinbaugh; Ricardo Quarrie; Fuchun Yang; M A Hassan Talukder; Jay L Zweier; Juan A Crestanello
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-26
Journal Detail:
Title:  The Journal of surgical research     Volume:  164     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-24     Completed Date:  2010-12-22     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  175-81     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure
Coronary Vessels / pathology,  physiopathology
Female
Heart / physiology*,  physiopathology
Heart Rate
Ischemic Postconditioning / methods*
Male
Myocardial Infarction / pathology*,  physiopathology
Myocardial Ischemia / physiopathology*
Rats
Rats, Sprague-Dawley
Reperfusion Injury / prevention & control
Sex Characteristics
Ventricular Dysfunction, Left / physiopathology
Ventricular Function, Left
Grant Support
ID/Acronym/Agency:
HL38324/HL/NHLBI NIH HHS; HL63744/HL/NHLBI NIH HHS; HL65608/HL/NHLBI NIH HHS; R01 HL038324/HL/NHLBI NIH HHS; R01 HL038324-20/HL/NHLBI NIH HHS
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