| Ischemia hypothermia improved contractility under normothermia reperfusion in the model of cultured cardiomyocyte. | |
| | |
MedLine Citation:
|
PMID: 22535380 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Though mild hypothermia displays an optimistic alleviation of contractive failure in the ischemia/reperfusion myocardium, we still lacked answers to many questions about its potential mechanisms. Our hypothesis is that hypothermia (32°C) induced in ischemia can ease mitochondrial injury resulting in improvement of myocardial contractility even under the condition of a normothermic reperfusion. Fifty newly born 1-2 d Sprague-Dawley rats were executed and the primary cardiomyocytes were obtained and cultivated in vitro. Myocytes were randomized into three groups and then subjected to ischemia either at 32°C or 37°C, both prior to undergoing reperfusion at 37°C. Contractility was presented as frequency and velocity. Ultrastructural alterations of cardiomyocytes and mitochondrion underwent semi-quantitative analysis with transmission electron microscopy and respiratory function of mitochondria was further assessed simultaneously. During cooling ischemia and following reperfusion, cardiomyocytes acquired a more immediate restoration to baseline level and had a significant difference as compared with those in normothermia (P < 0.05). Furthermore, hypothermia preserved the ultrastructure of myocytes and mitochondrion after ischemia. However, measurement on Heart Injury Score and form factor revealed no differences after 2-h reperfusion either in hypothermia or normothermia. On the contrary, the surface area and respiratory function of mitochondrion in reperfusion differed significantly in both groups (P < 0.05) which had an accordance with the variation on contractile performance. Hypothermia only induced in ischemia can bring contractility benefit even under a normothermia reperfusion in cultured cardiomyocytes. |
| | |
Authors:
|
Heng Li; Xiangshao Fang; Zhengfei Yang; Yue Fu; Yu Wang; Jinlang Wu; Tao Yu; Zitong Huang |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-26 |
Journal Detail:
|
Title: In vitro cellular & developmental biology. Animal Volume: - ISSN: 1543-706X ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-26 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Emergency Department, Sun-Yat-Sen Memorial Hospital, Guangzhou, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transcriptional response of Medicago truncatula sulphate transporters to arbuscular mycorrhizal symb...
Next Document: Graphenes prepared by Staudenmaier, Hofmann and Hummers methods with consequent thermal exfoliation ...