| Irreversible optical clearing of sclera by dehydration and cross-linking. | |
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MedLine Citation:
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PMID: 21055804 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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This study manipulates both clear cornea and opaque sclera by two dehydration processes for revealing the relationship between altered tissue structures and change in optical functions. In contrast to the high levels of light scattering in dehydrated tissues by critical point dry, a simple dehydration at 4-8 °C effectively and significantly improved their visible-light transmission, even in the sclera, with accompanying dense fiber packing. Further improvement in visible-light transmission, from 40-50% to 80-90%, has been achieved by flatting tissue surface with cover glasses during dehydration at low temperature. Such optical clearing of sclera by dehydration is reversible. However, chemical cross-linking effectively stabilizes their densely packed microscopic structures and visible-light transmission at over 50% irreversibly, even at wet conditions. Interestingly, the repetition of both low temperature dehydration/cross-linking treatments effectively reduced the required amounts of cross-linking reagents to keep a high transparency. Wet transparent cross-linked sclera can also show a characteristic strong tensile strength. Furthermore, rabbit corneal epithelium has regenerated on the transparent sclera with cross-linking in vitro. |
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Authors:
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Yuji Tanaka; Akira Kubota; Masayuki Yamato; Teruo Okano; Kohji Nishida |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-04 |
Journal Detail:
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Title: Biomaterials Volume: 32 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2010-11-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 1080-90 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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