| Irreversible inhibition of soybean lipoxygenase-1 by hydroperoxy acids as substrates. | |
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MedLine Citation:
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PMID: 1910308 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Soybean lipoxygenase-1 was irreversibly inactivated by various peroxy acids containing a cis,cis-1,4-pentadiene group. Among these compounds, 15(S)-hydroperoxyeicosatetraenoic acid (15(S)-HPETE)2 was found to be the most effective in the inactivation of lipoxygenase. Although the prior exposure of 15(S)-HPETE to hemoglobin abolished the inhibitory effect of 15(S)-HPETE, the simultaneous inclusion of hemoglobin potentiated the inactivation of lipoxygenase by 15(S)-HPETE alone. Interestingly, the potentiating effect of hemoglobin was observed only in the incubations with peroxy acids possessing the cis,cis-1,4-pentadiene. In either the presence or the absence of hemoglobin, it was commonly observed that the enzyme inactivation, which was maximal at pH 10, was significantly protected by tocopherol, but neither by mannitol nor ethanol, and that the inclusion of arachidonic acid or linoleic acid prevented the enzyme inactivation. Based on these results, it is suggested that the selective inactivation of lipoxygenase by these peroxy acids may be due to unstable intermediates produced from hydroperoxy acids bound to the active site of lipoxygenase. |
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Authors:
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M R Kim; D E Sok |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 288 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1991 Jul |
Date Detail:
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Created Date: 1991-10-24 Completed Date: 1991-10-24 Revised Date: 2001-11-02 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 270-5 Citation Subset: IM |
Affiliation:
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Department of Food and Nutrition, Chungnam National University, Daejeon, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Leukotrienes
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chemistry,
pharmacology* Lipid Peroxides / chemistry, pharmacology* Lipoxygenase Inhibitors* Soybeans / enzymology Structure-Activity Relationship Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Leukotrienes; 0/Lipid Peroxides; 0/Lipoxygenase Inhibitors; 67675-14-3/15-hydroperoxy-5,8,11,13-eicosatetraenoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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