Document Detail


Irreversible electroporation of the pancreas in swine: a pilot study.
MedLine Citation:
PMID:  20590911     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Irreversible electroporation (IRE) is a novel, non-thermal method of tissue ablation using short pulses of high-voltage DC current to ablate tissue.
METHODS: Irreversible electroporation of the pancreas was performed in four domestic female swine using two monopolar probes spaced 9-15 mm apart. Ninety pulses of 1500 V/cm were delivered for each ablation.
RESULTS: All animals survived for their designated times of 2 h (n = 1), 2 days (n = 1) and 14 days (n = 2), respectively. No procedure-related complications occurred. Three animals in which probes had been spaced at intervals of 10 ± 1 mm showed evidence of irreversible ablation, with ablation height ranging from < 10 mm to 21 mm and ablation width ranging from < 10 mm to 16 mm by gross appearance and triphenyltetrazolium chloride (TTC) staining. The only animal in which probes had been spaced at intervals of 15 mm did not show evidence of irreversible ablation at 2 weeks. This may be secondary to the wider probe spacing and relatively low voltage, which results in a mostly reversible form of electroporation without cell death.
CONCLUSIONS: Irreversible electroporation appears to be a safe method for pancreas tissue ablation. Staining with TTC can predict the zone of IRE ablation within 2 h of treatment.
Authors:
Kevin P Charpentier; Farrah Wolf; Lelia Noble; Brody Winn; Murray Resnick; Damian E Dupuy
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  HPB : the official journal of the International Hepato Pancreato Biliary Association     Volume:  12     ISSN:  1477-2574     ISO Abbreviation:  HPB (Oxford)     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-01     Completed Date:  2011-02-09     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  100900921     Medline TA:  HPB (Oxford)     Country:  England    
Other Details:
Languages:  eng     Pagination:  348-51     Citation Subset:  IM    
Affiliation:
Department of Surgery, Rhode Island Hospital and the Warren Alpert School of Medicine, Brown University, Providence, RI, USA. kcharpentier@lifespan.org
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MeSH Terms
Descriptor/Qualifier:
Ablation Techniques* / adverse effects
Amylases / blood
Animals
Biological Markers / blood
Electroporation*
Feasibility Studies
Female
Lipase / blood
Necrosis
Pancreas / enzymology,  pathology,  surgery*
Pilot Projects
Sus scrofa
Time Factors
Chemical
Reg. No./Substance:
0/Biological Markers; EC 3.1.1.3/Lipase; EC 3.2.1.-/Amylases
Comments/Corrections

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