| Irreversible deacylation of plasma membrane phospholipids by the combined action of Mg2+ and a long-chain acyl-CoA synthetase inhibitor in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 16233300 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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A long-chain acyl-CoA synthetase (Faap) inhibitor, adenosine 5'-hexadecylphosphate (AMPC16), caused lethal plasma membrane damage to Saccharomyces cerevisiae cells as reflected by the leakage of cytoplasmic K+ into medium in which Mg2+ was supplemented at 10 mM. AMPC16 did not interfere with the de novo synthesis of phospholipids using acetyl-CoA as a starting material, but the AMP analog accelerated the liberation of long- chain fatty acids from phospholipids with the aid of exogenous Mg2+, suggesting that the role of this divalent cation is to maximize the rate of the acyl group turnover. This Mg2+-dependent fungicidal effect of AMPC16 was similarly observed with a mutant lacking any of the phospholipase B (Plbp) isozymes, whereas it was mostly suppressed in a phospholipase C (Plc1p) deletion mutant in which the liberation of fatty acids from phospholipids was completely prevented. These results suggest that Plc1p is normally functional for phospholipid reconstitution in exponentially growing cells and enhancement of its activity by exogenous Mg2+ could be a cause of the irreversible deacylation of plasma membrane phospholipids when their reacylation is blocked at the step of activation of long-chain fatty acids by one of the Faap isozymes. |
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Authors:
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Keiji Nakayama; Taro Nakamura; Makoto Taniguchi; Toshio Tanaka |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 94 ISSN: 1389-1723 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2002 |
Date Detail:
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Created Date: 2005-10-19 Completed Date: 2005-11-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 258-63 Citation Subset: - |
Affiliation:
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Department of Bio- and Geoscience, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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