Document Detail

Irradiation effect on osteoclastogenesis stimulated by breast cancer cells.
MedLine Citation:
PMID:  21799342     Owner:  NLM     Status:  In-Data-Review    
To examine the effects of carbon ion and gamma ray irradiation on cancer-induced osteoclastogenesis, mouse calvaria MC3T3-E1 cells were cultured with conditioned medium from irradiated and non-irradiated MCF7 human breast cancer cells. The authors examined RANKL and OPG mRNA expression in osteoblastic MC3T3-E1 cells following treatment with conditioned MCF7 medium. Co-cultured MC3T3-E1 and bone marrow cells treated with conditioned medium from irradiated MCF7 cells showed decreased numbers of osteoclasts, assessed using TRAP staining. Conditioned medium from control MCF7 cells elevated the RANKL/OPG mRNA ratio in MC3T3-E1 cells; this effect was suppressed by carbon ion irradiation of the MCF7 cells. These data demonstrate that indirect interactions between breast cancer cells and MC3T3-E1 cells induce osteoclastogenesis in vitro through modulation of RANKL expression and that this process is suppressed by carbon ion irradiation.
Masahiko Sawajiri; Yuji Nomura; Samir Banik; Ryo Nishikiori; Yoshiko Sonoda; Keiji Tanimoto
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Health physics     Volume:  101     ISSN:  1538-5159     ISO Abbreviation:  Health Phys     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-07-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985093R     Medline TA:  Health Phys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-64     Citation Subset:  IM    
* Department of Oral and Maxillofacial Radiology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hirsoshima 734-8553, Japan; † Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
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