| Iron(III) binding in DNA solutions: complex formation and catalytic activity in the oxidation of hydrazine derivatives. | |
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MedLine Citation:
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PMID: 1647885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A strong interaction between iron(III) and calf thymus DNA at pH 7.4 was demonstrated in the present study by separation of the complex by column chromatography and by the slow kinetics of iron(III) removal from DNA by disodium-1,2-dihydroxybenzene-3,5-disulfonate (Tiron). An equilibrium constant of 2.1 x 10(14) was calculated by measurements of bound iron(III) by flame atomic absorption spectroscopy and assuming a one iron to two nucleotide stoichiometry. Graphic analysis of the interaction however, indicated that DNA has two binding sites for iron(III) characterized by a stoichiometry of one iron to 12 nucleotides and one iron to 2 nucleotides, and association constants of 4.8 x 10(12) and 2.3 x 10(11), respectively. The DNA-iron(III) complex isolated by column chromatography was shown to catalyze the oxidation of both 2-phenylethylhydrazine and methylhydrazine by spin-trapping experiments with alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN). By contrast, oxidation of 1,2-dimethylhydrazine was not catalyzed. Catalysis of 2-phenylethylhydrazine oxidation was confirmed by oxygen consumption studies. The results suggest that iron chelated to DNA may be significant in DNA damage induced by oxidizable chemicals. |
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Authors:
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L E Netto; A M Ferreira; O Augusto |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chemico-biological interactions Volume: 79 ISSN: 0009-2797 ISO Abbreviation: Chem. Biol. Interact. Publication Date: 1991 |
Date Detail:
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Created Date: 1991-08-08 Completed Date: 1991-08-08 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0227276 Medline TA: Chem Biol Interact Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-14 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Instituto de Química, Sao Paulo, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Catalysis Cattle Centrifugation Chromatography, Gel DNA / chemistry* Electron Spin Resonance Spectroscopy Ferric Compounds / chemistry* Hydrazines / chemistry* Hydrogen-Ion Concentration Nitrogen Oxides / chemistry Oxidation-Reduction Phenelzine / chemistry Pyridines Solutions |
| Chemical | |
Reg. No./Substance:
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0/Ferric Compounds; 0/Hydrazines; 0/Nitrogen Oxides; 0/Pyridines; 0/Solutions; 0/alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone; 51-71-8/Phenelzine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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